Matrix Metalloproteases: Methods and Protocols

PART I Expression and Purification of Matrix Metalloproteases

 1. Expression and Purification of Matrix Metalloproteinases in Escherichia coli

Krishna K. Singh, Ruchi Jain, Harini Ramanan, and Deepak K. Saini

2. Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli

Charles A. Galea

3. Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris

Dagmar Schlenzig and Stephan Schilling

PART II Structural Characterization of Matrix Metalloproteases

 4. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction

Elena Decaneto, Wolfgang Lubitz, and Hideaki Ogata

5. Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins

Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, and Steven R. Van Doren

6. Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)

PART III Computational Simulations of Matrix Metalloproteases

 7. Molecular Dynamics Studies of Matrix Metalloproteases

Natalia Díaz and Dimas Suárez

PART IV Determining Matrix Metalloprotease Substrate Specificity

 8. Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates

Maciej J. Stawikowski, Anna M. Knapinska, and Gregg B. Fields

9. Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples

10. Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini

Zon W. Lai and Oliver Schilling

11. Mapping the Substrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis

Colin A. Kretz

PART V Detection of Matrix Metalloproteases

 12. Detection of Matrix Metalloproteinases by Zymography

Rajeev B. Tajhya, Rutvik S. Patel, and Christine Beeton

13. Imaging Matrix Metalloproteases in Spontaneous Colon Tumors:  Validation by Correlation with Histopathology<

Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, and Margie L. Clapper

Tanya Singh, B. Jayaram, and Olayiwola Adedotun Adekoya

16. A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality

Jennifer S. Myers, Joan Hare, and Qing-Xiang Amy Sang

PART VII Matrix Metalloproteases as Biomarkers

 17. Matrix Metalloproteases as Biomarkers of Disease

Fernando Luiz Affonso Fonseca, Beatriz da Costa Aguiar Alves, Ligia Ajaime Azzalis, and Thaís Moura Gáscon Belardo