The Nuclear Receptor Superfamily: Methods and Protocols

The Nuclear Receptor Superfamily- AtThirty.- Lipid Homeostasis and Ligands forLiver X Receptors: Identification and Characterization.- Useof Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for NovelInhibitors of the Androgen Receptor.- Useof BRET to Study Protein-Protein Interactions in vitro and in vivo.- StudyingSteroid Receptor Complexes in the Cellular Environment.- Post-translationalModifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to IdentifyChromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated byAndrogens.- Methods for Identifying andQuantifying Mrna Expression of Androgen Receptor Splicing Variants in ProstateCancer.- HarvestingHuman Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- Invivo Imaging of Nuclear Receptor Transcriptional Activity.- Developmentand Characterisation of Cell-Specific Androgen Receptor Knockout Mice.