Bioluminescence: Methods and Protocols

 Label-free Cell Phenotypic Identificationof D-Luciferin as an Agonist for GPR35.- Synthetic BioluminescentCoelenterazine Derivatives.- Molecular Cloning of SecretedLuciferases from Marine Planktonic Copepods.-  How to Fabricate FunctionalArtificial Luciferases for Bioassays.- QuantumYield Determination Based on Photon Number Measurement, Protocols for FireflyBioluminescence Reactions.- Bioluminescent Ligand-ReceptorBinding Assays for Protein or Peptide Hormones.- Bioluminogenic Imaging of AminopeptidaseN in vitro and in vivo.-  Firefly Luciferase-basedSequential Bioluminescence Resonance Energy Transfer (BRET)-FluorescenceResonance Energy Transfer (FRET) Protease Assays.- Monitoring Intracellular pH Changewith a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast andMammalian Cells.- A Protein-Protein InteractionAssay FlimPIA Based on the Functional Complementation of Mutant FireflyLuciferases.- Single-chain Probes for IlluminatingAndrogenicity of Chemicals.- MulticolorImaging of Bifacial Activities of Estrogens.-  CircularPermutation Probes for Illuminating Phosphorylation of Estrogen Receptor.-  Fabricationof Molecular Strain Probes for Illuminating Protein-Protein Interactions.- AnALuc-based Molecular Tension Probe for Sensing Intramolecular Protein-Protein Interactions.- Live Cell Bioluminescence Imagingin Temporal Reaction of G Protein-coupled Receptor for High-throughput Screeningand Analysis.-  Imaging Histone Methylations inLiving Animals.-  Preparation and Assay of Simple Light off Biosensor Based on ImmobilizedBioluminescent Bacteria for General Toxicity Assays.- InVivo Bioluminescent Imaging of ATP-BindingCassette Transporter-Mediated Efflux at the Blood-Brain Barrier.- Theranostic Imaging of CancerGene Therapy.-  Development of a Multicolor BioluminescenceImaging Platform to Simultaneously Investigate Transcription Factor NF- B Signalingand Apoptosis .- A Multichannel BioluminescenceDetermination platform for Bioassays.- A Bioluminescence Assay Systemfor Imaging Metal Cationic Activities in Urban Aerosols.- LuminescenceImaging: (a) Multi-color Visualization of Ca²⁺ Dynamics in DifferentCellular Compartments and (b) Video-rate Tumour Detection in a Freely Moving Mouse.-  Photon Counting System for High-sensitivityDetection of Bioluminescence at Optical Fiber End.