Split Inteins: Methods and Protocols

1. Affinity Purification of Proteins in Tag-Free Form: Split-Intein-Mediated Ultra-Rapid Purification (SIRP)
Dongli Guan and Zhilei Chen

2. Purification of Microbially Expressed Recombinant Proteins via a dual ELP Split Intein System
Changhua Shi, Tzu-Chiang Han, and David W. Wood

3. Intracellular Production of Cyclic Libraries with SICLOPPS
Eliot L/ Osher and Ali Tavassoli

4. Recombinant Expression of Cyclotides Using Split Inteins
Krishnappa Jagadish and Julio A. Camarero

5. Ribosomal Synthesis of Thioether-Bridges Bicyclic Peptides
Nina Bionda and Rudi Fasan

6. Preparation of Semisynthetic Peptides Macrocycles Using Split Inteins
Shubhendu Palei and Henning D. Mootz

7. Semisynthesis of Membrane-Attached Proteins Using Split Inteins
Stefanie Hackl, Alanca Schmid, and Christian F.W. Becker

8. Protein Chemical Modification Inside Living Cells Using Split Inteins

9. Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology
Dongsheng Liu and David Cowburn

1 0. Segmental Isotope Labeling of Insoluble Proteins for Solid-State NMR by Protein Trans-Splicing
Tobias Schubeis, Madhu Nagaraj, and Christiane Ritter

11. Split-Intein Triggered Protein Hydrogels
Miguel A. Ramirez and Zhilei Chen

12. A Recessive Pollination Control System for Wheat-Based on Intein-Mediated Protein Splicing
Mario Gils

13. Conditional Toxin Splicing Using a Split Intein System
Spencer C. Alford, Connor O'Sullivan, and Perry L. Howard

14. Photocontrol of the Src Kinase in mammalian Cells with a Photocaged Intein
Wei Ren and Hui-Wang Ai

15. LOV2-Controlled Photoactivation of Protein Trans-Splicing
Anam Qudrat, Abdullah Mosabbir, and Kevin Truong

16. A Cassette Approach for the Identification of Intein Insertion Sites
Tim Sonntag

17. Computational Prediction of New Intein Split Sites
Yi-Zong Lee, Wei-Cheng Lo, and Shih-Che Sue