Cohesin and Condensin: Methods and Protocols

Part I: In Vitro and In Vivo Systems to Dissect Molecular Mechanisms of Cohesin Regulation in Mitosis and Meiosis

1. A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts
Keishi Shintomi and Tatsuya Hirano

2. An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex
Yasuto Murayama and Frank Uhlmann

3. Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl
Ge Zheng, Zhuqing Ouyang, and Hongtao Yu

4. Detection of Cohesin SUMOylation In Vivo
Marcelino Bermúdez-López and Luis Aragón

5. Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans
Aaron F. Severson

6. Protein and Chromosome Analysis in Mammalian Meiocytes
Michelle Stevense, Rolf Jessberger, and Attila Tóth

Part II: The Roles of Cohesin in Gene Regulation, Chromatin Interactions, Ribosome Biogenesis, and DNA Repair

7. Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex
Kerstin S. Wendt

8. Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq
Dale Dorsett and Ziva Misulovin

9. A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis
Jinbo Fan, Hui Jin, and Hong-Guo Yu

10. Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing
Misty R. Peterson, Omar Hamdani, and Rohinton T. Kamakaka

11. Zebrafish as a Model to Study Cohesin and Cohesinopathies
Akihiko Muto and Thomas F. Schilling

12 Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase
Preksha Gupta, Thais Lavagnolli, Hegias Mira-Bontenbal, and Matthias Merkenschlager

13. Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins
Dong-Hwan Kim, Andrew C. Box, Hua Li, and Jennifer L. Gerton

14. The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair
Xiangduo Kong, Alexander R. Ball, Jr., and Kyoko Yokomori

Part III: Dissection of Condensin Function in Mitotic Chromosome Condensation and Gene Regulation



15. A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells Christoph Schiklenk, Boryana Petrova, and Christian H. Haering

16. ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells
Toyonori Sakata, Katsuhiko Shirahige, Takashi Sutani