Potassium Channels: Methods and Protocols

Foreword...
Preface...
Table of Contents...
Contributing Authors...

1. Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation
Kang Wang

2. Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Heming Wei, Jianjun Wu, and Zhenfeng Liu

3. Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane using Surface Biotinylation
Jing-Syuna Ruan and Pei-Chun Chen

4. Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome
Qiuming Gong and Zhengfeng Zhou

5. Probing Subunits Interactions in KATP Channels using Photo-Crosslinking via Genetically Encoded p-azido-L-phenylalanine
Prassana Devaraneni, Emily A. Rex, and Show-Ling Shyng

6. Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization
Xu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Huanjie Shao, Hongmei Wu, and Yitao Qi

7. Simultaneous Real-Time Measurement of the beta-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on beta-Cell Function
Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, and David A. Jacobson

8. Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations
Balamurugan Kandasamy and Show-Ling Shyng

9. Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters
C. David Weaver

10. Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions
Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, and Ichio Shimada

11. Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes
Josefina del Mármol, Robert A. Rietmeijer, and Stephen G. Brohawn

12. Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations
Qiansen Zhang and Huaiyu Yang

13. Studying Structural Dynamics of the Potassium Channels by Single-Molecule FRET
Shizhen Wang, Joshua Brettmann, and Colin G. Nichols

14. Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters
Kimberly Matulef and Francis I. Valiyaveetil

15. Combinatorial Assembly of Lumitoxins
David Nedrud and Daniel Schmidt

16. Characterization of MC4R Regulation of the Kir7.1 Channel using the Tl+ Flux Assay
Michael J. Litt, Roger D. Cone, and Masoud Ghamari-Langroudi

17. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies
David J. Posson, Radda Rusinova, Olaf S. Andersen, and Crina M. Nimigean

18. In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish
Jui-Yi Hsieh and Diane M. Papazian

19. Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes
Robin Y. Kim and Harley T. Kurata

20. Random Spherically Constrained Single-Particle (RSC) Method to Study Voltage-Gated Ion Channels
Liguo Wang

21. CW-EPR Spectroscopy and Site-Directed Spin Labeling to Study the Structural Dynamics of Ion Channels
Cholpon Tilegenova, Benjamin W. Elberson, D. Marien Cortes, and Luis G. Cuello

22. Ion Binding to Transport Proteins using Isothermal Titration Calorimetry
Shian Liu and Steve W. Lockless

23. Building Atomic Models of the Ion Channels Based on Low Resolution Electron Microscopy Maps and Homology Modeling
Valery Novoseletsky, Olfat Malak, Gildas Loussouarn, and Olga S. Sokolova

24. Studying Kv Channels Function using Computational Methods
Audrey Deyawe, Marina A. Kasimova, Lucie Delemotte, Gildas Loussouarn, and Mounir Tarek