Cellular Quiescence: Methods and Protocols

1. Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches

Wai-Kin So and Tom H. Cheung

2. An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts

Mithun Mitra, Linda D. Ho, and Hilary A. Coller

3. Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

4. Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells

Hassan Azari, Loic P. Deleyrolle, and Brent A. Reynolds

5. Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System

Alexandra Chicheportiche, Martial Ruat, François D. Boussin, and Mathieu Daynac

6. Determination of Histone 2B-Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells

Kevin R. Hughes and Yashwant R. Mahida

7. Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation

Katie A. Matatall, Claudine S. Kadmon, and Katherine Y. King

8. Cell Cycle Analysis by Mass Cytometry

Gregory K. Behbehani

9. Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells

Marla M. Spain, Sarah G. Swygert, and Toshio Tsukiyama

10. Identifying Quiescent Stem Cells in Hair Follicles

Christine N. Rodriguez and Hoang Nguyen

11. Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells

Caroline E. Brun, Yu Xin Wang, and Michael A. Rudnicki

12. Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence

Kai Yang and Hongbo Chi

13. Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells

Chun Shik Park and H. Daniel Lacorazza

14. Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation

Emilie Jalbert and Eric M. Pietras

15. A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment

Johanna M. Buschhaus, Kathryn E. Luker, and Gary D. Luker

16. Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis

Surabhi Srivastava, Hardik Gala, Rakesh K. Mishra, and Jyotsna Dhawan

17. Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assay and RNA Immunoprecipitation (RIP)

Holger Bierhoff

18. Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells

Syed I.A. Bukhari, Samuel S. Truesdell, and Shobha Vasudevan

19. Genome-Wide Identification of Transcription Factor Binding Sites in Quiescent Adult Neural Stem Cells

Shradha Mukherjee and Jenny Hsieh

20. Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling

Jungeun Sarah Kwon, Xia Wang, and Guang Yao