Molecular Motors: Methods and Protocols

Preface... Table of Contents... Contributing Authors...
1. Cellular and Nuclear Forces: An Overview Bidisha Sinha, Arikta Biswas, and Gautam V. Soni
Part I Membrane Motors
2. The Bacterial Flagellar Rotary Motor in Action Nicholas J.S. Delalez and Judith P. Armitage
3. Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase Ganna O. Krasnoselska and Thomas Meier
Part II Cytoskeletal Motors
4. Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly Guillaume Romet-Lemonne, Bérengère Guichard, and Antoine Jégou
5. Engineering Synthetic Myosin Filaments using DNA Nanotubes Ruth F. Sommese and Sivaraj Sivaramakrishnan
6. Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy Noriyuki Kodera and Toshiro Ando
7. High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates Keith J. Mickolajczyk and William O. Hancock
8. Multicolor Tracking of Molecular Motors at Nanometer Resolution Sara Wichner and Ahmet Yildiz
9. High-Speed Optical Tweezers for the Study of Single Molecular Motors L. Gardini, A. Tempestini, F.S. Pavone, and M. Capitanio
10. Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy Matthew Batchelor, Marcin Wolny, Marta Kurzawa, Lorna Dougan, Peter J. Knight, and Michelle Peckham
Part III Nucleic Acids Motors
11. The Role of Supercoiling in the Motor Activity of RNA Polymerases Annick Lesne, Jean-Marc Victor, Edouard Bertrand, Eugenia Basyuk and Maria Barbi
12. Single-Molecule FRET Analysis of Replicative Helicases Seung-Jae Lee, Salman Syed, and Taekjip Ha
13. Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy Pauline Dupaigne, Eliana Moreira Tavares, Olivier Piétrement, and Eric Le Cam
14. DNA Organization and Segregation Super-Resolved Diego I. Cattoni, Jean-Bernard Fiche, Antoine Le Gall, and Marcelo Nollmann
15. Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase Belén Martínez-García, Antonio Valdés, Joana Segura, Ofelia Díaz-Ingelmo, and Joaquim Roca
16. Single Molecule Angular Optical Trapping for Studying Transcription under Torsion Jie Ma, Chuang Tan, and Michelle D. Wang
17. Anisotropy-Based Nucleosome Repositioning Assay Koan Briggs, Gada Al-Ani, Allen Eastlund, and Christopher J. Fischer
18. Remodeling and Repositioning of Nucleosomes in Nucleosomal Arrays Johanna Ludwigsen, Nicola Hepp, Henrike Klinker, Sabrina Pfennig, and Felix Mueller-Planitz
19. Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers Damian J. delToro and Douglas E. Smith
20. Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers Nicholas Keller, Damian J. delToro, and Douglas E. Smith
21. Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor Farzin Haque, Hui Zhang, Shaoying Wang, Chun-Li Chang, Cagri Savran, and Peixuan Guo