Mouse Oocyte Development: Methods and Protocols

1. Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse

Stephany El-Hayek, Qin Yang, and Hugh J. Clarke

2. In vitro Culture of Mouse Oocytes for Meiotic Maturation

Ewa Borsuk and Jacek Z. Kubiak

3. In vitro Maturation and Culture of Human Oocytes

Jennifer R Gruhn, Stine Gry Kristensen, Claus Yding Andersen, and Eva R Hoffmann

4. Pronuclear Transfer in Human Oocytes

Louise Hyslop

5. In vitro Fertilization of Mouse Oocyte

Agathe Chaigne

6. Profiling Maternal mRNA Translation during Oocyte Development

Joao P. Sousa Martins and Marco Conti

7. Transcriptome Profiling of Single Mouse Oocytes

8. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation

9. Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation

Jessica Greaney, Zhe Wei, and Hayden Homer

10. Triple-colour Live Imaging of Mouse Oocytes

Aurélien Courtois, Petr Solc, and Tomoya S. Kitajima

11. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes

Elvira Nikalayevich, Nora Bouftas, and Katja Wassmann

12. Manipulating Cohesin Levels in Live Mouse Oocytes

Anna Szydlowska, Sabrina Ladstätter, and Kikuë Tachibana

13. Optogenetic Manipulation of Mouse Oocytes

Takashi Akera, David M. Chenoweth, and Michael A. Lampson

14. Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin

Greg FitzHarris

15. Photoactivation of Actin in Mouse Oocyte

Maria Almonacid

16. Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes

Isma Bennabi and Marion Manil-Ségalen

17. Micropipette Aspiration of Oocytes to Assess Cortical Tension

Janice P. Evans and Douglas N. Robinson