Functional Genomics in Medicago truncatula: Methods and Protocols

Chapter 1. Introduction

1.1. Legumes and agriculture sustainability

1.2. Grain and forage legumes: model systems used in developmental studies

1.3. Functional genomics in M. truncatula: applications in developmental studies

References

Abstract

1. Introduction

2. The M. truncatula genome

3. Genomic data availability in M. truncatula

References

Chapter 3. Chemical mutagenesis in M. truncatula: ethyl methanesulphonate (EMS) and ethyl-nitrosourea (ENU)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

Chapter 4. Physical mutagenesis in M. truncatula: g-rays and Fast Neutron Bombardment (FNB)

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

Chapter 5. T-DNA insertional mutagenesis and activation tagging in M. truncatula

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

Chapter 6. Tnt1 insertional mutagenesis in M. truncatula

Abstract

1. Introduction

3. Methods

4. Notes

References

Chapter 7. Target Induced Local Lesions IN Genomes (TILLING) in M. truncatula

Abstract

2. Materials

3. Methods

4. Notes

References

Chapter 8. Transient post-transcriptional gene silencing in M. truncatula: Virus-Induced Gene Silencing (VIGS)

1. Introduction

2. Materials

3. Methods

4. Notes

References

Chapter 9. Stable post-transcriptional gene silencing in M. truncatula: RNAi

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

References

Abstract

1. Introduction

2. Materials

3. Methods

4. Notes

Chapter 11. Gene silencing quantification in M. truncatula: fluorescence in situ
hibridization (FISH)

1. Introduction

2. Materials

3. Methods

4. Notes

References

Chapter 12. Functional genomics to study seed development in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of seed development in M. truncatula

References

Chapter 13. Functional genomics to study plant architecture and branching in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of plant architecture and branching in M. truncatula

References

Chapter 14. Functional genomics to study compound leaf development in M. truncatula: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of compound leaf development in M. truncatula

References

Abstract

1. Introduction

2. Functional genomics and genetic control of root and nodule development in M. truncatula

References

Chapter 16. Functional genomics and M. truncatula flowering time: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of floral development in M. truncatula

References

Chapter 17. Functional genomics and M. truncatula floral development: an overview

Abstract

1. Introduction

2. Functional genomics and genetic control of floral development in M. truncatula

References

Chapter 18. Functional genomics and M. truncatula fruit development: an overview

1. Introduction

2. Functional genomics and genetic control of fruit development in M. truncatula

References

Chapter 19. Emerging tools in functional genomics of M. truncatula: the CRISPR-Cas9 system

Abstract

1. Introduction

2. Editing the M. truncatula genome: targeted mutagenesis and gene editing using the CRISPR-Cas9 system

References

Chapter 20. Future prospects in functional genomics of legumes

2. New legumes created through genome engineering versus transgenic ones carrying foreign DNA in their genomes: will be more acceptable for the consumers?

References