Recombinant Protein Expression in Mammalian Cells: Methods and Protocols

1.         Transient Gene Expression in Suspension HEK293-EBNA1 Cells

Denis L'Abbé, Louis Bisson, Christian Gervais, Eric Grazzini and Yves Durocher

2.         Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells

Joanna Pieprzyk, Samuel Pazicky and Christian Löw

3.         PEI-Mediated Transient Gene Expression in CHO Cells

Yashas Rajendra

4.         Stable Expression by Lentiviral Transduction of Cells

Natascha Gödecke, Hansjörg Hauser and Dagmar Wirth

5.         Inducible Protein Production in 293 Cells using the piggyBac Transposon System

Iacovos P. Michael and Andras Nagy

6.         Recombinant CHO Cell Pool Generation using piggyBac Transposon System

Sowmya Balasubramanian

7.         Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression

Cristina Parola, Derek Mason, Andreas Zingg, Daniel Neumeier and Sai T. Reddy

8.         Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor

David I. Fisher

9.         CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors

David L. Hacker, Laurence Durrer and Soraya Quinche

10.       Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension

Dominique Monteil and Jeffrey Kuan

11.       Continuous and Integrated Expression and Purification of Recombinant Antibodies

Sebastian Vogg, Moritz Wolf and Massimo Morbidelli

12.       High Throughput Transfection of HEK293 Cells for Transient Protein Production

Tia A. Arena, Peter D. Harms and Athena W. Wong

13.       Microfluidic Transfection for High-Throughput Mammalian Protein Expression

Kristina Woodruff and Sebastian J. Maerkl

14.       Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production

Sarah Inwood, Michael Betenbaugh, Madhu Lal and Joseph Shiloach

15.       Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells

Kevin Kellner, Ankur Solanki, Thomas Amann, Nga T Lao and Niall Barron

16.       Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells

17.       Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)

Daniel Blessing, Nicole Déglon and Bernard L. Schneider

18.       Considerations in the Use of Codon Optimization for Recombinant Protein Expression 

Vincent P. Mauro and Stephen A. Chappell

19.       Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells

Lena Thoring and Stefan Kubick