Foodborne Bacterial Pathogens: Methods and Protocols

Part I: Bacterial Pathogen Detection and Quantification in Food 1. Accelerated Sample Preparation for Fast Salmonella Detection in Poultry ProductsEduardo Ximenes, Seockmo Ku, Lori Hoagland, Michael R. Ladisch
2. Direct or DNA Extraction-free Amplification and Quantification of Foodborne PathogensMaggie R. Williams and Syed A. Hashsham
3. The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial PathogensAlejandro Garrido-Maestu, David Tomás Fornés, and Marta Prado Rodríguez
4. Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial DetectionTanja Kostic and Levente Bodrossy
5. Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic DevicesNobuyasu Yamaguchi
6. Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of BacteriaMatthew Dunne and Martin J. Loessner
7. EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenesPalmiro Poltronieri, Elisabetta Primiceri, and Rajeswaran Radhakrishnan
Part II: Phenotypic and Metabolic Characterization of Foodborne Pathogens8. Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing ConditionsOlivier Habimana
9. Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation Thomas Brauge, Graziella Midelet-Bourdin, and Christophe Soumet
10. Isolation of Bacterial RNA from Foods Inoculated with PathogensDeepti Tyagi, Autumn L. Kraft, and Teresa M. Bergholz
11. The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal StressDenise Schrama and Maria Leonor Faleiro
12. Identification of Putative Biomarkers Specific to Food-borne Pathogens Using MetabolomicsSnehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe, David J. Beale, Konstantinos A. Kouremenos, and Enzo A. Palombo
13. Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells Florence Dubois-Brissonnet
14. High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens Alexis Canette, Julien Deschamps, and Romain Briandet
15. Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae Macarena A. Varas, Javiera Orti z-Severi n, Andre s E. Marcoleta, Carlos A. Santiviago , Miguel L. Allende, and Francisco P. Cha vez 
16. Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen InfectionFrancisco P. Chávez and Andrea Calixto
Part III: NGS and Modeling Approaches
17. Utilizing the public GenomeTrakr Database for Foodborne Pathogen TracebackRuth E. Timme, Maria Sanchez Leon, and Marc W. Allard
18. The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq PlatformKristina M. Feye and Steven C. Ricke
19. Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun MetagenomicsArnaud Bridier
20. Modelling Growth of Listeria and Lactic Acid Bacteria in Food EnvironmentsP. Dalgaard and O. Mejlholm
21. Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars Marton Olbei, Robert A. Kingsley, Tamas Korcsmaros, and Padhmanand Sudhakar