Oligodendrocytes: Methods and Protocols

Preface... Table of Contents... Contributing Authors...
1. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System Sueli Marques, David van Bruggen, and Gonçalo Castelo-Branco
2. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron
3. Myelin: Methods for Purification and Proteome Analysis Michelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner
4. In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells Dario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey
5. Isolation and Culture of Oligodendrocytes Marie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller, and Mikael Simons
6. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation Marie E. Bechler
7. Oligodendrocyte-Neuron Myelinating Co-Culture Matthew Swire and Chalres ffrench-Constant
8. Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier Andrew A. Jarjour and Diane L. Sherman
9. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices Sylvia Agathou and Ragnhildur Thóra Káradóttir
10. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord Sowmya Sekiza and Anna Williams
11. Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination Linde Kegel, Maria Rubio, Rafael Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons
12. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination Jill M. Williamson, David A. Lyons, and Rafael G. Almeida
13. A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation Marja J. Karttunen and David A. Lyons
14. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis Abdelkrim Mannioui and Barnard zalc
15. Conditional Mutagenesis in Oligodendrocyte Lineage Cells Sandra Goebbels and Klaus-Armin Nave
16. Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage Jaime Eugenin-von Bernhardi and Leda Dimou
17. The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination Maria Traka
18. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease John N. Mariani, Lisa Zou, and Steven A. Goldman
19. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes Graham R. Campbell and Don J. Mahad
20. Transmission Electron Microscopy of Oligodendrocytes and Myelin Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Mogusia Sadowski, and Wiebke Möbius
21. Toxin-Based Models to Investigate Demyelination and Remyelination Christopher E. McMurran, Chao Zhao, and Robin J M. Franklin
22. Magnetic Resonance techniques for Imaging White Matter Cassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg