Intracellular Lipid Transport: Methods and Protocols

2.       Use of new probes to detect sphingolipids

4.       Update on the strategy to follow the movement of DAG and PA lipids.

5.       TIRF microscopy to measure lipid movement in and out the PM with fluorescent-tagged probe

6.       BRET & FRET approaches to measure intracellular lipid transport.

7.       Measure of dynamics of native lipid species (caged-lipids) in cells.

8.       Cell fractionation and lipidomics to measure lipid movements in organelles.

9.       PS and PE transport at ER-mitochondria contact sites and inside mitochondria

10.   Lipid transport and endosomal-lysosomal compartment.

11.   Measurement of neutral lipid and sterol accumulation in lipid droplets.

12.   Lipid transport in plants (ER-chloroplast contact sites).

13.   Measure of glycolipids transport.

14.   Identification of putative lipid-transporter by sequence analysis.

15.   Identification of lipid transporter and their ligand by purification affinity and denaturating mass spectrometry

16.   Use of click-chemistry to identify novel lipid transporters and their ligands.

17.   In vitro real-time measure of lipid exchange by ORP/Osh protein - Guillaume Drin et al.

18.   Measure of lipid transport by liposome separation approaches

19.   In silico examination of membrane binding and lipid release activity of an LTP

20.    Advanced approaches to measure lipid scramblase/flippase activity

21.   Additionnal : mathematical approaches for modeling lipid transport in cells