Neisseria meningitidis: Methods and Protocols

1. An Overview of Neisseria meningitides

            Sarah Hollingshead and Christoph M. Tang

2. Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR

            Ala-Eddne Deghmane, Eva Hong, and Muhamed-Kheir Taha

3. Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

            Nadja Heidrich, Saskia Bauriedl, and Christoph Schoen

            Holly Bratcher, Odile Harrison, and Martin Maiden

5. Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis

            Luke R. Green, Richard D. Haigh, and Christopher D. Bayliss

6. Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis

            Freda E.C. Jen, Kate L. Seib, and Aimee Tan

7. Sample Preparation for Proteomic Analysis of Neisseria meningitides

            Benjamin L. Schulz

8. Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance

            Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Christopher J. Day, Michael P. Jennings, and Kate L. Seib

9. The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

            Emily A. Kibble, Mitali Sarkar-Tyson, Geoffrey W. Coombs, and Charlene M. Kahler

10. In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells

            Brandon J. Kim and Alexandra Schubert-Unkmeir

11. Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

            Kay O. Johswich and Scott D. Gray-Owen

            Jay Lucidarme, Jennifer Louth, Kelly Townsend-Payne, and Ray Borrow

13. Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines

            Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez, and Reinaldo Acevedo

14. Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine

            Giuseppe Boccadifuoco, Brunella Brunelli, Elena Mori, Mauro Agnusdei, Claudia Gianfaldoni, and Marzia Monica Giuliani

15. Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides

            Jakob Loschko, Karen Garcia, David Cooper, Michael Pride, and Annaliesa Anderson

16. Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis

            Caroline Cayrou and Christopher D. Bayliss