Adipose-Derived Stem Cells: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
1.Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose TissueAnnie C. Bowles, Alan Tucker, and Bruce A. Bunnell

2.A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant TimeframeJoel A. Aronowitz, Ryan A. Lockhart, and Cloe S. Hakakian

3.Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem CellsChristina L. Rettinger, Kai P. Leung, and Rodney K. Chan

4.The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem CellsAnne-Laure Hafner, Tala Mohsen-Kanson, and Christian Dani

5.Methylcellulose Based Thermally Reversible HydrogelsAnoosha Forghani and Ram Devireddy
6.Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, and Lauren E. Flynn

7.Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells Anthony D. Foster, Nicholas Clark, and Thomas A. Davis

8.High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered SubstitutesDominique Mayrand and Julie Fradette
 9.Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery Pietro Gentile and Valerio Cervelli 

10.Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction Pinar Yilgor Huri, Jordana Gilbert-Honick, Justin Morrissette-McAlmon, Sarah Somers, and Warren L. Grayson

11.Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based AnalysisGail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, and Z. Elizabeth Floyd

12.Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, and Mikhail G. Kolonin

13.Isolation of Human Adipose-Derived Stem Cells from LipoaspiratesJie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, and Jeffrey M. Gimble

14.Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method Jie Li, Hui Li, and Weidong Tian

15.Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation Carmel Fargason, Nan Zhang, and Mandi J. Lopez

16.Soft Tissue ReconstructionKacey G. Marra

17.Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain Rachel C. Nordberg, Josie C. Bodle, and Elizabeth G. Loboa

18.Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells Shahensha Shaik and Ram Devireddy 

19.Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma   Satoshi Tajima, Morikuni Tobita, and Hiroshi Mizuno

20.Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes  Hui Yu, Margo Emont, Heejin Jun, and Jun Wu