Macrophages: Methods and Protocols

Preface...

Table of Contents...

Contributing Authors...

1. Culture of Human Monocyte-Derived Macrophages

Aoife Kelly, Aleksander M. Grabiec, and Mark A. Travis

2. A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages

Chandrayana Mukherjee, Christine Hale, and  Subhankar Mukhopadhyay

3. Growing Murine Bone Marrow-Derived Macrophages

Anaïs Assouvie, Lisa P. Daley-Bauer, and Germain Rousselet

4. Immortalization of Murine Bone Marrow-Derived Macrophages

5. Isolation and Identification of Murine Serous Cavity Macrophages

Calum C. Bain and Stephen J. Jenkins

6. Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies

Shaikh M. Atif, Sophie L. Gibbings, and Claudia V. Jakubzick

7. Isolation and Phenotyping of Adult Mouse Microglial Cells

Kathleen Grabert and Barry W. McColl

8. Isolation and Phenotyping of Bone Marrow Macrophages

Marion Chalot

Vanessa Petit

10. Phenoytyping Tumor Associated Macrophages

R. Ian Cumming and Yen-Rei A. Yu

11. Activating Murine Macrophages In Vitro

Elizabeth Dalby

12. Polarizing Macrophages In Vitro

Xuan Huang, Yong Li, Mingui Fu, and Hong-Bo Xin

13. Viral Replication Assay in Bone Marrow-Derived Macrophages

Linda Roback and Lisa P. Daley-Bauer

14. Macrophage Bactericidal Assays

Mourad Aribi

15. Quantitative Phagocytosis Assays in Primary and Cultured Macrophages

Fernando Montaño, Sergio Grinstein, and Roni Levin

16. Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM)

17. Chromatine Immunoprecipitation in Macrophages

Germain Rousselet

18. Transfecting Macrophages

Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Wittig, and Stefan Lorkowski

19. In Vitro Migration Assays

Lewis Taylor, Carlota Recio, David R. Greaves, and Asif J. Iqbal

20. Cytokine-Induced Acute Inflammatory Monarticular Arthritis

Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook

Dominik Ruckerl

22. Genetic Models of Macrophage Depletion

Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren

23. Depleting Macrophages In Vivo with Clodronate-Liposomes

Stéphanie G. Moreno

24. Cre Driver Mice Targeting Macrophages

Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren