Myelin: Methods and Protocols
Lieferzeit: 7-14 Werktage
- Artikel-Nr.: 10376886
Beschreibung
Preface
Table of Contents
Contributing Authors
Part I Imaging Myelin and Biochemical Analysis
1.Electron Microscopy for the Analysis of Peripheral Nerve Myelin
Cinzia Ferri, Angelo Quattrini, and Maurizio D'Antonio
2.Immunohistochemical Analysis of Myelin Structures
M. Thetiot, S.A. Freeman, and A. Desmazières
3.Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish
Stavros Vagionitis and Tim Czopka
4.Lipidomics Profiling of Myelin
Chunyan Wang, Juan Pablo Palavicini, and Xianlin Han
5.Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry
Roberto Fernández, Jone Garate, Beatriz Abad, Begoña Ochoa, and José A. Fernández
Akihiro Ishii, David Han, and Rashmi Bansal
Part II Cell Culture Systems
7.Isolation and Purification of Primary Rodent Schwann Cells
Marta Palomo Irigoyen, Miguel Tamayo Caro, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, and Ashwin Woodhoo
8.Isolation, Expansion, and Maturation of Oligodendrocyte Lineage Cells Obtained from Rat Neonatal Brain and Optic Nerve
Maria Victoria Sánchez-Gómez, Mari Paz Serrano, Elena Alberdi, Fernando Pérez-Cerdá, and Carlos Matute
9.DRG Neuron/Schwann Cells Myelinating Co-Cultures
Carla Taveggia and Alessandra Bolino
10.Neuron/Oligodendrocyte Myelination Co-Culture
Yi Pang, Kimberly Simpson, José Javier Miguel-Hidalgo, and Renate Savich
11.Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination
Mohammad Amin Sherafat, Robert A. Hill, and Akiko Nishiyama12.Modeling PNS and CNS Myelination Using Microfluidic Chambers
Adrien Vaquié, Alizée Sauvain, and Claire Jacob
13.Modeling CNS Myelination Using Micropillar Arrays
Nan-Xing Huang, Yun-An A. Shen, and Feng Mei
14.A Central Nervous System Axonal Myelination Assay for High-Throughput Screening
Karen Lariosa-Willingham and Dmitri Leonoudakis
15.Analyzing Autophagic Flux in Nerve Cultures
Miguel Tamayo Caro, Marta Palomo Irigoyen, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, and Ashwin Woodhoo
16.Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro
Agata Blasiak, Sudip Nag, and In Hong Yang
Part III Modeling Demyelinating Disorders and In Vivo Targeting Strategies
Mercedes Giralt, Amalia Molinero, and Juan Hidalgo
18.Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin
Chenyi Wang and Mark R. Kotter
David G. Gonsalvez, SangWon Yoo, Georgina A. Craig, Rhiannon J. Wood, Jessica L. Fletcher, Simon S. Murray, and Junhua Xiao
20.Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve
Xin-peng Dun and David B. Parkinson
21.In Vivo Introduction of Transgenes into Mouse Sciatic Nerve Cells Using Viral Vectors
Gerben Van Hameren, Sergio Gonzalez, Ruani N. Fernando, Claire Perrin-Tricaud, and Nicolas Tricaud
22.Intrathecal Delivery of Viral Vectors for Gene Therapy
Alexia Kagiava and Kleopas A. Kleopa
23.Efficient Gene Transfer to Myelinating Schwann Cells in the Rodent Sciatic Nerve Using In Vivo Electroporation
Daisuke Ino and Masamitsu Iino