Progranulin: Methods and Protocols

Preface... Table of Contents... Contributing Authors...
Part I Introduction
1. A Brief Overview of Progranulin in Health and Disease Andrew Bateman, Siu Tim Cheung, and Hugh P.J. Bennett
Part II Analytical and In Vitro Methods for the Study of Progranulin Biology
2. Chromatographic Methods for the Purification of Granulin Peptides Andrew Bateman, Babykumari Chitramuthu, and Hugh P.J. Bennett
3. Methods for Expression and Purification of Biologically Active Recombinant Progranulin Chun Yu Zhao and Andrew Bateman
4. Large-Scale Generation of Recombinant Granulin Peptides in E. coli Dmitri Tolkachev
5. Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging Dmitri Tolkatchev
6. Data Mining: Applying the AD&FTD Mutation Database to Progranulin Marc Cruts and Christine Van Broeckhoven
Part III Methods to Investigate the Role of Prgranulin in Cancer
7. Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases Ginette Serrero and David Hicks
8. Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker Ginette Serrero and David Hicks
9. Analysis of Progranulin-Mediated Akt and MAPK Activation Shi-Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonio Belfiore, Renato V. Iozzo, and Andrea Morrione
10. Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models Chi Wai Yip, Phyllis F.Y. Cheung, Nicholas C.L. Wong, Sze Wai Fung, and Siu Tim Cheung
11. Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features Phyllis F.Y. Cheung and Siu Tim Cheung
12. Methods to Study the Role of Progranulin in the Tumor Microenvironment Moshe Elkabets and Samuel Brook
Part IV Animal Models to Study the Role of Progragulin in Health and Disease
13. Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo using Caenorhabditis elegans James Julian Doyle and J. Alex Parker
14. The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response Tsung-Yuan Hsu, Victoria J. Butler, and Aimee W. Kao
15. Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function Babykumari P. Chitramuthu and Hugh P.J. Bennett
16. Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice Jan Boddaert, Hans Wils, and Samir Kumar-Singh
17. Methods to Investigate the Protection Againts Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain Jackalina M. Van Kampen
18. The Interaction between Progranulin with Sortilin and the Lysosome Xiaolai Zhou, Peter M. Sullivan, Daniel H. Paushter, and Fenghua Hu
19. Methods to Study the Role of Progranulin in Preimplanatation Mouse Embryo Development Laura Diaz-Cueto, Fabian Arechavaleta-Velasco, and George L. Gerton
20. Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-Inflammatory Activity of Progranulin in Inflammatory Arthritis Jian-Iu Wei and Chuan-ju Liu
21. Methods for Studying the Function of Progranulin in Atherosclerosis using Both Knockout Mice Models and In Vitro Studies Tohru Ohama, Ryota Kawase, Yasushi Sakata, and Shizuya Yamashita
22. Methods to Investigate the Roles of Prgranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models Huishi Toh, Eugene Daniels, and Andrew Bateman