TRP Channels: Methods and Protocols

1. Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels

            Pau Doñate-Macián, Alba Crespi-Boixader, and Alex Perálvarez-Marín

2. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

            Appu K. Singh, Luke L. McGoldrick, and Alexander I. Sobolevsky

3. Cryo-Electron Microscopy of TRP Channels

            Amrita Samanta, Taylor E.T. Hughes, and Vera Y. Moissenkova-Bell

4. Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation

            Verónica I. Dumit, Michael Köttgen, and Alexis Hofherr

5. Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels

            Victoria Oakes and Carmen Domene

6. Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

            Elvira de la Peña and Ana Gomis

7. Fluorescence-Based Functional Assays for Ca²⁺ Permeable ThermoTRP Channels

            Celia Cordero-Sánchez, Irene Mudarra-Fraguas, and Asia Fernández-Carvajal

8. High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons

            Jörg Isensee and Tim Hucho

9. Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels

            Beiying Liu and Feng Qin

10. TRP Channels Reconstitution in Lipid Bilayers

            Eleonora Zakharian

11. Methods for Investigating TRP Channel Gating

            Osvaldo Alvarez, Karen Castillo, Emerson Carmona, Carlos Gonzalez, and Ramon Latorre

12. In Silico Approaches for TRP Channel Modulation

            Magdalena Nikolaeva Koleva and Gregorio Fernández-Ballester

13. Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction

            Tracey Pirali, Ubaldina Galli, Marta Serafini, Alessia Griglio, Armando A. Genazzani, and Gian Cesare Tron

14. In Vivo Methods to Study ThermoTRP Channels in Rodents

            Sara González-Rodríguez