Plant Innate Immunity: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
1Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae StrainsJay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, and Kee Hoon Sohn
2Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in plantaMaxime Escouboué and Laurent Deslandes
3Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeAKarl J. Schreiber and Jennifer D. Lewis
4A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor FunctionsMustafa Adhab and James E. Schoelz
5Quantification of Extracellular ATP in Plant Suspension Cell CulturesSowmya R. Ramachandran, Sonika Kumar, and Kiwamu Tanaka
6Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen InteractionsGerit Bethke and Jane Glazebrook
7Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in TomatoSupriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, and Pallob Kundu
8Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIMMaxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, and Laurent Deslandes 
9Analysis of DNA Methylation Profile in Plants by Chop-PCRPratiti Dasgupta and Shubho Chaudhuri
10Change in Nucleosome Dynamics during Stress Responses in PlantsAmit Paul and Shubho Chaudhuri
11Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow CytometryLeiyun Yang, Zhixue Wang, and Jian Hua
12A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light EnvironmentsDaniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, and Emmanuel Liscum 
13Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae Conner J. Rogan and Jeffrey C. Anderson
14 Quantification of Cauline Leaf Abscission in Response to Plant PathogensO. Rahul Patharkar
15Methods for Replicating Leaf Vibrations Induced by Insect HerbivoresSabrina C.J. Michael, Heidi A. Appel, and Reginald B. Cocroft
16Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via HydathodesMarieke van Hulten, Sayantani Chatterjee, and Harrold A. van den Burg
17The Apple Fruitlet Model System for Fire Blight DiseaseSara M. Klee, Judith P. Sinn, and Timothy W. McNellis
18Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R ProteinsSharon Pike, Walter Gassmann, and Jianbin Su
19Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression SystemsAnkita Shrestha, Ahamed Khan, and Nrisingha Dey
20Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in PlantsAhamed Khan, Ankita Shrestha, and Nrisingha Dey
21Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant GrowthRobert J. Kremer
22A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance Eduardo Balsanelli, Vânia Carla Pankievicz, Valter Antonio Baura, Fábio de Oliveira Pedrosa, and Emanuel Maltempi de Souza
Subject Index List...