Chimera Research: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
Part I Human Donor Cell Types: Potency and Position 
1. Frontiers of PluripotencyAlejandro De Los Angeles
2. Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions Alejandro De Los Angeles, Daiji Okamura, and Jun Wu
3.Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem CellsRyohichi Sugimura
4. Cancer Stem Cells: Concepts, Challenges and Opportunities for Cancer TherapyMay Yin Lee, Rajshekhar R. Giraddi, and Wai Leong Tam
5. Informed Consent Issues for Cell DonorsInsoo Hyun
Part II Non-Human Hosts: Species and Developmental Stages  6. Chick Models and Human-Chick Organizer GraftsIain Martyn, Tatiane Y. Kanno, and Ali H. Brivanlou 7. The Engraftment of Lentiviral Vector-Transduced Human CD34+ Cells into Humanized MiceYoon-Sang Kim, Matthew Wielgosz, and Byoung Ryu 8. Pig Chimeric Model with Human Pluripotent Stem Cells Cuiqing Zhong, Jun Wu, and Juan Carlos Izpisua Belmonte
9. Embryonic Chimeras with Human Pluripotent Stem CellsAlejandro De Los Angeles, Masahiro Sakurai, and Jun Wu 10. Neural Stem Cell Transplantation into a Mouse Model of Stroke Alejandro De Los Angeles
11. Ethical Standards for Chimera Research OversightInsoo Hyun Part III New Directions and Controversies
12. Ethical Considerations in Crossing the XenobarrierSebastian Porsdam Mann, Rosa Sun, and Göran Hermerén
13. Neurological Chimeras and the Moral StaircaseDaniel Counihan
14. Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells Nilam Sinha, Eoin C. Whelan, and Ralph L. Brinster
15. Human-Monkey Chimeras for Modeling Human Disease: Opportunities and ChallengesAlejandro De Los Angeles, Insoo Hyun, Stephen R. Latham, John D. Elsworth, and D. Eugene Redmond Jr.