Enzyme-Mediated Ligation Methods

Preface...Table of Contents...Contributing Authors...
1.Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug ConjugatesRémy Gébleux, Manfred Briendl, Ulf Grawunder, and Roger R. Beerli
2.Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)Hejia Henry Wang and Andrew Tsourkas
3.Cyclizing Disulfide-Rich Peptides using Sortase AAkello J. Agwa, David J. Craik, and Christina I. Schroeder
4.Chemo-Enzymatic Synthesis of  Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1Marcel Schmidt and Timo Nuijens
5.Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)Igor Rupniewski and David Rabuka
6.Butelase 1-Mediated Ligation of Peptides and ProteinsXinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, and James P. Tam
7.Trypsiligase-Catalyzed Peptide and Protein LigationSandra Liebscher and Frank Bordusa
8.Site-Specific Antibody-Drug Conjugation Using Microbial TransglutaminaseStephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, and Nicolas Rasche
9.Tailoring Activity and Selectivity of Microbial Transglutaminase Lukas Deweid, Olga Avrutina, and Harald Kolmar
10.SpyLigase-Catalyzed Modification of AntibodiesVanessa Siegmund, Birgit Piater, Frank Fischer, and Harald Kolmar
11.Peptide Cyclization Catalyzed by Cyanobactin MacrocyclasesWael E. Houssen
12.In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinisKaren S. Harris, Simon Poon, Pedro Quimbar, and Marilyn A. Anderson
13.Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed LigationJan Grünewald, Ansgar Brock, and Bernhard H. Geierstanger
14.Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and ImmobilizationJoseph G. Plaks and Joel L. Kaar
15.BioID as a Tool for Protein Proximity Labeling in Living CellsRhiannon M. Sears, Danielle G. May, and Kyle J. Roux
16.N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live CellsSamuel H. Ho and David A. Tirrell
17.Tubulin Tyrosine Ligase-Mediated Modification of ProteinsMarcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P.R. Hackenberger, Dominik Schumacher, and Jonas Helma
18.Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of TyrosineJorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, and Floria L. van Delft