ADAMTS Proteases: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
1. ADAMTS Proteins: Concepts, Challenges, and ProspectsSuneel S. Apte
2. Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing TechnologyCarlos Peris-Torres, Orlando Serrano, María del Carmen Plaza-Calonge and Juan Carlos Rodríguez-Manzaneque
3. Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins using Cell-Based AssaysSteven John Berardinelli and Robert S. Haltiwanger
4. Challenges and Solutions for Purification of ADAMTS Proteases: An Overview Alain C. Colige
5. Purification of Native or Recombinant ADAMTS2 and Procollagen I Cleavage AssayAlain C. Colige
6. ADAMTS7: Recombinant Protein Expression and PurificationRens de Groot
7. Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted MutantsMilan M. Fowkes and Ngee H. Lim
8. Expression and Preparation of Recombinant Reelin and ADAMTS-3 ProteinsTakao Kohno, Himari Ogino, Yuko Yamakage, and Mitsuharu Hattori
9. Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the BrainHimari Ogino, Yuko Yamakage, Mihoshi B. Yamashita, Takao Kohno, and Mitsuharu Hattori
10. Chemical Modification of Proteoglycanases with BiotinSalvatore Santamaria
11. Analysis of Aggrecanase Activity using Neoepitope AntibodiesSalvatore Santamaria and Kazuhiro Yamamoto
12. Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4Mukti Singh, Stuart A. Cain, and Clair Baldock
13. Purification of Recombinant ADAMTSL2 Mukti Singh and Clair Baldock
14. Expression Analysis by RNAscope(TM) In Situ Hybridization  Timothy J. Mead and Suneel S. Apte
15. Analysis of ADAMTS Effects on Cell Adhesion and MigrationJordi Lambert and Dylan R. Edwards
16. Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin MicrofibrilsDirk Hubmacher
17. Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton Timothy J. Mead 
18. Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS ProteinsValentin Nelea and Dieter P. Reinhardt
19. Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins Rongmo Zhang, Heena Kumra, and Dieter P. Reinhardt
20. Visualization of Perineuronal Nets in Central Nervous System Tissue SectionsLuke Souter and Jessica C. F. Kwok
 21. Visualization and Quantification of Pericellular MatrixTimothy J. Mead and Suneel S. Apte
22. Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP ActivityChun-Yao Yang, Linda Troeberg, and Simone D. Scilabra
23. Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS SubstratesRahel Schnellmann and Ruth Chiquet-Ehrismann
24. Mapping the N-Terminome in Tissue Biopsies by PCT-TAILSLouise Bundgaard, Simonas Savickas, and Ulrich auf dem Keller