Stem Cells and Aging: Methods and Protocols

1. Assessing Muscle Stem Cell Clonal Complexity during Aging

            Matthew T. Tierney, Michael J. Stec, and Alessandra Sacco

2. Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies

            Eusebio Perdiguero, Victoria Moiseeva, and Pura Muñoz-Cánoves

3. Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle

            Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler, and Julia von Maltzahn

4. Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue

            Meenakshi Gaur, Marek Dobke, and Victoria V. Lunyak

5. Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures

            Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, and Francis Rodier

6. Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells

            Courtney R. Ogando, Gilda A. Barabino, and Yueh-Hsun Kevin Yang

7. Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis

            Klemen Camernik, Janja Marc, and Janja Zupan

8. Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells

            Klemen Camernik and Janja Zupan

9. Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis

            Janja Zupan

10. 3D-Embedded Cell Cultures to Study Tendon Biology

            Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer, and Andreas Traweger

11. Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis

            Brooke Snetsinger, Christina Ferrone, and Michael J. Rauh

12. Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants

            Sherin Saheera and Renuka R. Nair

13. Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreER^T2/Rosa26YFP Mice

            Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, and Noël Ghanem

14. Infrared Spectroscopy and Imaging in Stem Cells and Aging Research

            Ceren Aksoy and  Feride Severcan

15. Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres

            Nedime Serakinci, Huseyin Cagsin, and Merdiye Mavis

16. Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry

            Klemen Camernik and Janja Zupan

17. Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry

            Rasmus Jentoft Boutrup

18. Methods for Detection of Autophagy in Mammalian Cells

            Bindu Singh and Sangeeta Bhaskar

19. Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

            Yohei Hayashi and Yasuhisa Matsui

20. Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer

            Dan-Ya Wu, Xia Zhang, and Yi-Liang Miao

21. Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)

            Harshini Surendran, Reena J. Rathod, and Rajarshi Pal

22. Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury

            Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko, and Cesar V. Borlongan

23. 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol

            Yuhui Lin

24. Isolation, Expansion, and Characterization of Wharton's Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments

            Shuh-Wen Aung, Noor Hayaty Abu Kasim, and Thamil Selvee Ramasamy

25. CRISPR Base Editing in Induced Pluripotent Stem Cells