Fundamental Approaches to Screen Abnormalities in Drosophila

Chapter 1. Preparation of normal food and modified food for Drosophila.- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly.- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol).- Chapter 4. Detection of fat within the Drosophila body.- Chapter 4.1. Nile red.- Chapter 4.2. Oil red.- Chapter 4.3. Sudan black.- Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells. -Chapter 5.1. DCF-DA staining.- Chapter 5.2. NBT assay.- Chapter 6. Methods to check the metabolic activity of fly.- Chapter 6.1. Floating assay.- Chapter 6.2. Survivability assay.- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy.- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy.- Chapter 8.1. Trypan blue stain.- Chapter 8.2. Prussian blue stain.- Chapter 8.3. Copper cell staining.- Chapter 8.4. Iron cell staining.- Chapter 9.Comet assay to detect severity of DNA damage.- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain.- Chapter 10. Analysis of channel proteins.- Chapter 11. Analysis of channel proteins.- Chapter 11.1. Temperature sensitivity assay.- Chapter 11.2. Sound-avoidance assay.- Chapter 11.3. Hygroscopicassay.- Chapter 12.Various behavioral assays in larva and adult Drosophila.- Chapter 12.1. Larva crawling.- Chapter 12.2. Adult climbing.- Chapter 12.3. Self-righting.- Chapter 12.4.Touch response test.- Chapter 12.5. Temperature sensitivity assay.- Chapter 12.6. Thermal avoidance behavior.- Chapter 12.7. Photosensitivity assay (Light- Dark).- Chapter 12.8. Light preference test.- Chapter 12.9. Chemotaxis assay.- Chapter 12.10. Courtship and mating.- Chapter 12.11. Grooming behavior. - Chapter 12.12. Aggression assay.- Chapter 12.13. Choice assay.- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR.- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM).- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM).