Single Cell Metabolism: Methods and Protocols

Preface... Table of Contents... Contributing Authors...
1. Single Cell Metabolomics by Mass Spectrometry Bindesh Shrestha
2. Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry Linwen Zhang, Linda L. Allworth, and Akos Vertes
3. Analysis of Lipids in Single Cells and Organelles using Nanomanipulation-Coupled Mass Spectrometry Mandy S. Phelps and Guido F. Verbeck
4. Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis Zhenwei Wei, Xiaochao Zhang, Xingyu Si, Xiaoyun Gong, Sichun Zhang, and Xinrong Zhang
5. Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells Ning Pan, Wei Rao, and Zhibo Yang
6. Applications of MicroArrays for Mass Spectrometry (MAMS) in Single Cell Metabolomics Alfredo J. Ibáñez and Ales Svatos
7. Laser Capture Microdissection - Liquid Vortex Capture - Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells John F. Cahill and Vilmos Kertesz
8. Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, and Bernhard Spengler
9. A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population Jasmin Krismer, Jens Sobek, Robert F. Steinhoff, Rolf Brönnimann, Martin Pabst, and Renato Zenobi
10. Sample Preparation and Analysis of Single Cells using High Performance MALDI FTICR Mass Spectrometry Bo Yang, Tina Tsui, Richard M. Caprioli, and Jeremy L. Norris
11. Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry Sylwia A. Stopka and Akos Vertes
12. Compact Quantum Dots for Quantitative Cytology Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, and Andrew M. Smith
13. Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos using Desorption Electrospray Ionization (DESI) Mass Spectrometry Christina R. Ferreira, Valentina Pirro, Alan K. Jarmusch, Clint M. Alfaro, and R. Graham Cooks
14. Spatial Mapping of Cellular Metabolites using DESI Ion Mobility Mass Spectrometry Anthony Midey, Hernando Olivos, and Bindesh Shrestha
15. Open-Source Software Tools, Databases, and Resources for Single Cell and Single Cell-Type Metabolomics Biswapriya B. Misra
16. Ten Major Future Challenges in Single Cell Metabolomics Bindesh Shrestha