Chimeric RNA: Methods and Protocols
Lieferzeit: 7-14 Werktage
- Artikel-Nr.: 10377177
Beschreibung
Part I: Identification and Characterization
1. Prediction, Characterization, and In Silico Validation of Chimeric RNAs
Sandeep Singh and Hui Li
2. Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope
Neetha Nanoth Vellichirammal, Abrar Albahrani, You Li, and Chittibabu Guda
3. Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras
Milana Frenkel-Morgenstern
4. Gene Fusion Discovery with INTEGRATE
Jin Zhang and Christopher A. Maher
5. Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit
Pankaj Vats, Arul M. Chinnaiyan, and Chandan Kumar-Sinha
Part II: Experimental Validation
6. Detection and Measurement of Chimeric RNAs by RT-PCR
Justin Elfman and Hui Li
7. Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells
Félix LaRoche-Johnston, Caroline Monat, and Benoit Cousineau
8. RNase Protection Assay
Jianzhu Zhao, Jun Tang, Justin Elfman, and Hui Li
9. Validation of Chimeric Fusion Peptides Using Proteomics Data
Sandeep Singh
10. NanoString nCounter Technology: High-Throughput RNA Validation
Angela Goytain and Tony Ng
Part III: Functional and Mechanistic Studies
11. Knockdown of Chimeric RNA by RNAi
Xinrui Shi, Fujun Qin, and Hui Li
12. Overexpression of Chimeric RNA by Retroviral Transduction
Hao Wu and Hui Li
13. Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization
Xinrui Shi, Fujun Qin, and Hui Li
14. Confirmation of Transcriptional Read-Through Events by RT-PCR
Xinrui Shi, Fujun Qin, and Hui Li
15. Validating Gene Fusion as the Source of Chimeric RNAs
Sachin Kumar Gupta, Jocelyn Duen-Ya Jea, and Laising Yen
Part IV: Translational Applications
16. Chimeric RNA and Exosomes-Based Liquid Biopsy
Xiurong Ke, Xiao Xiong, Yusheng Lin, and Hao Zhang
17. SMaRT for Therapeutic Purposes
Lisa M. Riedmayr
18. Case Study: Landscape of Chimeric RNAs in Bladder Cancer
Dingjun Zhu and Hui Li
19. Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells
Yue Tang, Fangxia Guan, and Hui Li
Eigenschaften
Breite: | 181 |
Gewicht: | 700 g |
Höhe: | 264 |
Länge: | 22 |
Seiten: | 262 |
Sprachen: | Englisch |
Autor: | Hui Li, Justin Elfman |