Protein'Protein Interactions: Methods and Applications

Part I. Overview Structural Basis of Protein-Protein Interactions Robert C. Liddington Quantitative Analysis of Protein-Protein Interactions Keith D. Wilkinson Part II. In Vitro Techniques Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire Circular Dichroism Analysis for Protein-Protein Interactions Norma J. Greenfield Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy Guanghua Gao, Jason G. Williams, and Sharon L. Campbell Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance John Northup Using Light Scattering to Determine the Stoichiometry of Protein Complexes Jeremy Mogridge Sedimentation Equilibrium Studies Ian A. Taylor, John F. Eccleston, and Katrin Rittinger Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions Sang-Hyun Park and Ronald T. Raines Fluorescence Polarization Assay to Quantify Protein-Protein Interactions Sang-Hyun Park and Ronald T. Raines Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot Randy A. Hall Glutathione-S-Transferase-Fusion-Based Assays for Studying Protein-Protein Interactions Haris G. Vikis and Kun-Liang Guan Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery William E. Pierceall, Lixin Zhang, and Dallas E. Hughes Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting Nick Loizos Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions Michael Mourez and R. John Collier Part III. Detecting Protein-Protein Interactions in Heterologous Systems A Bacterial Two-Hybrid System Based on Transcriptional Activation Simon L. Dove and Ann Hochschild Using the Yeast Two-Hybrid System to Identify Interacting Proteins John Miller and Igor Stagljar Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System Ilya G. Serebriiskii and Elena Kotova The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System Safia Thaminy, John Miller, and Igor Stagljar Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo Jae Woon Lee and Soo-Kyung Lee Co-Immunoprecipitation from Transfected Cells Shane C. Masters Part IV: Probing Protein-Protein Interactions in Living Cells Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) Brian Herman, R. V. Krishnan, and Victoria E. Centonze Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer Francis Ka-Ming Chan Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions Keith M. Berland Confocal Microscopy for Intracellular Co-localization of Proteins Toshiyuki Miyashita Mapping Biochemical Networks with Protein-Fragment Complementation Assays Ingrid Remy and Stephen W. Michnick In Vivo Protein Cross-Linking Fabrice Agou, Fei Ye, and Michel Véron Part V. Proteomics-Based Approaches Computational Prediction of Protein-Protein Interactions John C. Obenauer and Michael B. Yaffe Affinity Methods for