Membrane Protein Protocols: Expression, Purification, and Characterization

Part I. Expression of Membrane Proteins
Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
Lucy Waskell
Dihydroorotate Dehydrogenase of Escherichia coli
Kaj Frank Jensen and Sine Larsen
A General Approach for Heterologous Membrane Protein Expression in Escherichia coli: The Uncoupling Protein, UCP1, as an Example Alison Z. Shaw and Bruno Miroux
Expression of Membrane-Bound Iron-Sulfur Proteins
Dirk Schneider, Christian L. Schmidt, and Andreas Seidler
Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris
Chang-Hoon Han, Moritz Werder, Wilk von Gustedt, and Helmut Hauser
Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
U. Subrahmanyeswara Rao
Functional Expression of His-Tagged Rhodopsin Sf9 Insect Cells
Giel J.C.G.M. Bosman, Jenny van Oostrum, Githa Breikers, Petra H.M. Bovee-Geurts, Corne H.W. Klaassen, and Willem J. DeGrip
Part II. Detergent Selection in Membrane Protein Purification
Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
Darren Manley and Joe D. O'Neil
Solubilization of Chemokine Receptors from Cell Membranes
Robert Staudinger and Juan C. Bandrés
A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid: Acyl-CoA Acyltransferase (LAT)
Claus Kerkhoff and Volkhard Kaever
Part III. Membrane Protein Purification and Reconstitution
Purification of Omp50, a Minor Porin of Campylobacter jejuni
Jean Michel Bolla
Purification of Porins from Mycobacterium smegmatis
Christian Heinz, Eva Roth, and Michael Niederweis
Isolation of the Melanocortin 5 Receptor: From cDNA Sequence to Isolating an Integral Membrane Protein
Benjamin L.Clarke
Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography
Kentaro Hanada and Masahiro Nishijima
Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells
Kiyoshi Kawasaki and Masahiro Nishijima
Purification of Pancreastatin Receptor from Rat Liver Membranes
Víctor Sánchez-Margalet, José Santos-Alvarez, and Sandra Díaz-Troya
Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
Rajendra Prasad
Isolation of Lipid Raft-Associated Proteolipids
Jaime Millán, María C. de Marco, Mohammed Qaidi, Alicia Batista, Fernando Martín-Belmonte, and Miguel A. Alonso
Purification of Membrane-Bound Catechol-O-Methyltransferase
Maria João Bonifácio and Patrício Soares-da-Silva
Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
Da-Neng Wang, M. Joanne Lemieux, and Jonathan M. Boulter
Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase: A Putative PS Flippase
Jill V. Lyles, Kathleen Cornely-Moss, Christine M. Smith, and David J. Daleke
Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
Sathyanarayana N. Gummadi, Sigrún Hrafnsdóttir, Jane Walent, William E. Watkins, and Anant K. Menon
Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
Nicholas F. Brown
Part IV. Structural Analysis of Membrane Proteins
Crystallization in Lipidic Cubic Phases: A Case Study with Bacteriorhodopsin
Valentin I. Gordeliy, Ramona Schlesinger, Rouslan Efremov, Georg Büldt, and Joachim Heberle
Optical Biosensor Assay Using Retroviral Receptor Pseudotypes
Joseph