Trinucleotide Repeat Protocols

Part I. Introduction
Mouse Models of Triplet Repeat Diseases
Gillian P. Bates and David G. Hay
Part II. Analysis of Triplet Repeat DNAs and RNAs
Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis
Maria M. Krasilnikova and Sergei M. Mirkin
Genetic Analysis for Triplet Repeat Instability in Yeast
Michael J. Dixon, Saumitri Bhattacharyya, and Robert S. Lahue
Detection and Isolation of Trinucleotide Repeat Expansions Using the RED Method
Qiu-Ping Yuan and Martin Schalling
Analysis of Unstable Triplet Repeats Using Small-Pool Polymerase Chain Reaction
Mário Gomes-Pereira, Sanjay I. Bidichandani, and Darren G. Monckton
Real-Time RT-PCR for CTG Repeat-Containing Genes
Maria Eriksson
Part III. Detection and Analysis of Polyglutamine-Containing Proteins and Their Aggregates
Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms
Ali Khoshnan, Susan Ou, Jan Ko, and Paul H. Patterson
Using Antibodies to Analyze Polyglutamine Stretches
Elizabeth Brooks, Montserrat Arrasate, Kenneth Cheung, and Steven M. Finkbeiner
Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells
Noriko Hazeki and Ichiro Kanazawa
Part IV. Establishment of Animal and Cultured Cell Models for Trinucleotide Repeat Diseases
Caenorhabditis elegans as a Model System for Triplet Repeat Diseases
Cindy Voisine and Anne C. Hart
Monitoring Aggregate Formation in Organotypic Slice Cultures From Transgenic Mice
Donna L. Smith and Gillian P. Bates
The CGG Repeat and the FMR1 Gene
Violeta Stoyanova and Ben A. Oostra
Analysis of CTG Repeats Using DM1 Model Mice
Cédric Savouret, Claudine Junien, and Geneviève Gourdon
Lentiviral-Mediated Gene Transfer toModel Triplet Repeat Disorders
Etienne Régulier, Diana Zala, Patrick Aebischer, and Nicole Déglon
Mouse Tissue Culture Models of Unstable Triplet Repeats
Mário Gomes-Pereira and Darren G. Monckton
Part V. In Vivo Analysis of Trinucleotide Repeat Diseases
Neurotransmitter Receptor Analysis in Transgenic Mouse Models
Caroline L. Benn, Laurie A. Farrell, and Jang-Ho J. Cha
Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse Brain
Melissa W. Braveman, Alice S. Chen-Plotkin, George J. Yohrling, and Jang-Ho J. Cha
Techniques for Thick-Section Golgi Impregnation of Formalin-Fixed Brain Tissue
Tracie L. Moss and William O. Whetsell, Jr.
Assessment of Impaired Proteasomal Function in a Cellular Model of Polyglutamine Diseases
Nihar Ranjan Jana and Nobuyuki Nukina
Assessment of In Vitro and In Vivo Mitochondrial Function in Friedreich's Ataxia and Huntington's Disease
Anthony Schapira and Raffaele Lodi
Triplet Repeats and DNA Repair: Germ Cell and Somatic Cell Instability in Transgenic Mice
Irina V. Kovtun, Craig Spiro, and Cynthia T. McMurray
Oxidative Damage in Huntington's Disease
José Segovia and Francisca Pérez-Severiano
Index