DNA Viruses: Methods and Protocols

Part I. Viral Detection
Viral Detection
Feng Wang-Johanning and Gary L. Johanning
Quantitative Detection of Epstein-Barr Virus DNA in Clinical Specimens by Rapid Real-Time PCR Targeting a Highly Conserved Region of EBNA-1
Servi J. C. Stevens, Sandra A. W. M. Verkuijlen, and Jaap M. Middeldorp
Profiling of Epstein-Barr Virus Latent RNA Expression in Clinical Specimens by Gene-Specific Multiprimed cDNA Synthesis and PCR
Servi J. C. Stevens, Antoinette A. T. P. Brink, and Jaap M. Middeldorp
Quantitative Detection of Viral Gene Expression in Populations of Epstein-Barr Virus-Infected Cells In Vivo
Donna R. Hochberg and David A. Thorley-Lawson
Detection and Quantification of the Rare Latently Infected Cell Undergoing Herpes Simplex Virus Transcriptional Activation in the Nervous System In Vivo
Nancy M. Sawtell
Reporter Cell Lines for the Detection of Herpes Simplex Viruses
Szu-Hao Kung
Part II. Virus Structure and Imaging
Unraveling the Architecture of Viruses by High-Resolution Atomic Force Microscopy
Alexander J. Malkin, Marco Plomp, and Alexander McPherson
Studying the Structure of Large Viruses With Multiresolution Imaging
Carmen San Martín
Herpes Simplex Virus-Cell Interactions Studied by Low-Fading Contrasted Immunofluorescence
Helle Lone Jensen and Bodil Norrild
Herpes Simplex Virus-Cell Interactions Studied by Immunogold Cryosection Electron Microscopy
Helle Lone Jensen and Bodil Norrild
FTIR Microscopy Detection of Cells Infected With Viruses
Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, and Mahmoud Huleihel
Part III. Virus Entry
The JC Virus-Like Particle Overlay Assay
Hirofumi Sawa and Rika Komagome
Analysis of Fusion Using a Virus-Free Cell Fusion Assay
Marisa P. McShane and Richard Longnecker
Pseudovirions asSpecific Tools for Investigation of Virus Interactions With Cells
Martin Sapp and Hans-Christoph Selinka
Part IV. Gene Expression
Simultaneous In Situ Detection of RNA, DNA, and Protein Using Tyramide-Coupled Immunofluorescence
Brian A. Van Tine, Thomas R. Broker, and Louise T. Chow
Identification and Characterization of Herpesviral Immediate-Early Genes
Yan Yuan
Part V. Replication and Genome Maintenance
Methods for Measuring the Replication and Segregation of Epstein-Barr Virus-Based Plasmids
Priya Kapoor and Lori Frappier
DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins
Constandache Atanasiu, Larissa Lezina, and Paul M. Lieberman
Part VI. Pathogenesis
Pre-B-Cell Colony Formation Assay
Masato Ikeda and Richard Longnecker
Luciferase Real-Time Bioluminescence Imaging for the Study of Viral Pathogenesis
Gary D. Luker and David A. Leib
Part VII. Complex Cell Systems
Culturing Primary and Transformed Neuronal Cells for Studying Pseudorabies Virus Infection
Toh Hean Ch'ng, E. Alexander Flood, and Lynn William Enquist
Human Papillomavirus Type 31 Life Cycle: Methods for Study Using Tissue Culture Models
Frauke Fehrmann and Laimonis A. Laimins
Part VIII. Recombinant Genetics
Molecular Genetics of Herpesviruses: A Recombinant Technology Approach
Jason S. Knight, Subhash C. Verma, Ke Lan, and Erle S. Robertson
Molecular Genetics of DNA Viruses: Recombinant Virus Technology
Bernhard Neuhierl and Henri-Jacques Delecluse
Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis
Manfred Lee and Fenyong Liu
Construction of a Gene Inactivation Library for Bovine herpesvirus 1 Using Infectious Clone Technology
Timothy J. Mahony, Fiona M. McCarthy, Jennifer L. Gravel, and Peter L. Young
Selective Sile