Pichia Protocols

Table of Contents Chapter 1 - Introduction [REVIEW CHAPTER] Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER] Chapter 3 - DNA-Mediated Transformation Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity Chapter 6 - Initial processing and purification strategies Chapter 7 - Rapid screening of chromatography resins for the purification of proteins Chapter 8 - Characterization of O-linked saccharides on glycoproteins Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids Chapter 10 - N-linked glycan characterization of heterologous proteins Chapter 11 - Heavy labeling of recombinant proteins Chapter 12 - Selenomethionine labeling of recombinant proteins Chapter 13 - Deuterium labeling of proteins Chapter 14 - Classical genetic analysis Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI) Chapter 16 - Characterization of protein-protein interactions Chapter 17 - Localization of proteins and organelles using fluorescence microscopy Chapter 18 - Fluorescence microscopy and thin-section electron microscopy