RNA Silencing: Methods and Protocols

Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
Mikiko C. Siomi and Haruhiko Siomi
Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
John W. Pham and Erik J. Sontheimer
Preparation and Analysis of Drosha
Yoontae Lee and V. Narry Kim
RNA Interference in Caenorhabditis elegans
Nathaniel R. Dudley and Bob Goldstein
Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei
Christine E. Clayton, Antonio M. Estévez, Claudia Hartmann, Vincent P. Alibu, Mark Field, and David Horn
Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing
M. Florian Mette, Werner Aufsatz, Tatsuo Kanno, Lucia Daxinger, Philipp Rovina, Marjori Matzke, and Antonius J. M. Matzke
Strategies for the Design of Random siRNA Libraries and the Selection of Anti-GFP siRNAs
Mouldy Sioud and Sébastien Wälchli
Silencing Gene Expression with Dicer-Generated siRNA Pools
Jason W. Myers and James E. Ferrell, Jr.
Inhibition of Gene Expression In Vivo Using Multiplex siRNA
Sung-Suk Chae and Timothy Hla
Gene Silencing by a DNA Vector-Based RNAi Technology
Guangchao Sui and Yang Shi
Plasmid-Based RNA Interference: Construction of Small-Hairpin RNA Expression Vectors
Scott Q. Harper and Beverly L. Davidson
siRNA Delivery In Vivo
Mouldy Sioud
Peptide-Based Strategy for siRNA Delivery into Mammalian Cells
Federica Simeoni, May C. Morris, Frederic Heitz, and Gilles Divita
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
Mingjie Li and John J. Rossi
Exon-Specific RNA Interference: A Tool to Determine the Functional Relevance of Proteins Encoded by Alternatively Spliced mRNAs
Alicia M. Celotto, Joo-WonLee, and Brenton R. Graveley
Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs
Elisavet Maniataki, Maria Dels Angels De Planell Saguer, and Zissimos Mourelatos
Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
Marianthi Kiriakidou, Peter Nelson, Stella Lamprinaki, Anup Sharma, and Zissimos Mourelatos
High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes
Mouldy Sioud and Øystein Røsok
Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA
Peng Liu, Mary L. Stover, Alexander Lichtler, and David W. Rowe
Index