Plant Cell Culture Protocols

Part I. Introduction
An Introduction to Plant Cell Culture: Back to the Future
Victor M. Loyola-Vargas and Felipe Vázquez-Flota
History of Plant Tissue Culture
Trevor A. Thorpe
Part II. Cell Culture and Plant Regeneration: The Fundamentals
Pathogen and Biological Contamination Management: The Road Ahead
Alan C. Cassells and Barbara M. Doyle
Growth Measurements: Estimation of Cell Division and Cell Expansion
Gregorio Godoy-Hernández and Felipe A. Vázquez-Flota
Callus and Suspension Culture Induction, Maintenance, and Characterization
Alejandrina Robledo-Paz, María Nélida, Vázquez-Sánchez, Rosa María Adame-Alvarez, and Alba Estela Jofre-Garfias
Measurement of Cell Viability in In Vitro Cultures
Lizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-Ham
Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification
Qiaochun Wang and Avihai Perl
Somatic Embryogenesis in Picea Suspension Cultures
Claudio Stasolla
Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes
Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, and Richard Visser
Direct Somatic Embryogenesis in Coffea canephora
Francisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, and Victor M. Loyola-Vargas
Part III. Plant Propagation In Vitro
A New Temporary Immersion Bioreactor System for Micropropagation
Manuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, and Pedro Fuentes-Carrillo
Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro
Gabriela Fuentes, Carlos Talavera, Yves Desjardins, and Jorge M. Santamaría
Use of Statistics in Plant Biotechnology
Michael E. Compton
AnEfficient Method for the Micropropagation of Agave Species
Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, and Miguel Angel Herrera-Alamillo
Micropropagation of Endangered Plant Species
Zhihua Liao, Min Chen, Xiaofen Sun, and Kexuan Tang
Clonal Propagation of Softwoods
Trevor A. Thorpe, Indra S. Harry, and Edward C. Yeung
Part IV. Applications for Plant Protoplasts
Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora
Michael R. Davey, Paul Anthony, J. Brian Power, and Kenneth C. Lowe
Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts
Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, and Praveen K. Saxena
Production of Cybrids in Brassicaceae Species
Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, and Nikolay Kuchuk
Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants
Gary Tallman
Production of Interspecific Hybrid Plants in Primula
Juntaro Kato and Masahiro Mii
Part V. Protocols for Genomic Manipulation
Agrobacterium-Mediated Transformation of Petunia Leaf Discs
Ingrid M. van der Meer
Transformation of Wheat Via Particle Bombardment
Indra K. Vasil and Vilma Vasil
Chloroplast Transformation
Xiao-Mei Lu, Wei-Bo Yin, and Zan-Min Hu
The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers
César De los Santos-Briones and Teresa Hernández-Sotomayor
Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System
Zuo-yu Zhao and Jerry Ranch
Part VI. Accumulation of Metabolites in Plant Cells
Capsaicin Accumulation in Capsicum spp. Suspension Cultures
Neftalí Ochoa-Alejo
Isolation and Purification of Ribosome-Inactivating Proteins
Sang-Wook Park,