Tissue Proteomics: Pathways, Biomarkers, and Drug Discovery

Preface 1. Capabilities using 2-D DIGE in Proteomics Research: The New Gold Standard for Two-dimensional Gel Electrophoresis. Christine R. Rozanas and Stacey M. Loyland 2. Protein Carbohydrate Analysis: Gel-based Staining, Liquid Chromatography, Mass Spectrometry and Microarray Screening. Injae Shin, Alina D. Zamfir, and Bin Ye 3. Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry (SELDI-MS) for Protein and Peptide Profiling of Body Fluids. Audrey Gagnon, Qian Shi, and Bin Ye 4. A Novel Approach using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip technology) for Proteomic Profiling and Protein Identification. Sau-Mei Leung, and Rebecca L. Pitts 5. Automated Laser Capture Microdissection for Tissue Proteomics. Adrianna S. Rodriguez, Benjamin H. Espina, Virginia Espina, and Lance A. Liotta. 6. Tissue Microarrays: An overview. Rajiv Dhir 7. Frozen Protein Arrays. Stephen M. Hewitt and Robert A. Star. 8. Reverse Phase Protein Microarrays for Theranostics and Patient Tailored Therapy. Virginia Espina, Julia Wulfkuhle, Valerie S. Calvert, Lance A. Liotta, and Emanuel F. Petricoin III 9. Antibody Arrays for Determination of Relative Protein Abundances. Grigoriy S. Chaga 10. SH2 Domain Based Tyrosine Phosphorylation Array. Xin Jiang, Leslie Rose, Stephanie Han, and Xianqiang Li 11. Immunoregulomics: a serum autoantibody-based platform for transcription factor profiling. Oliver W. Tassinari, Margarita Aponte, Robert J. Caiazzo Jr., and Brian C.-S. Liu 12. The 'reverse capture' autoantibody microarray: an innovative approach to profiling the autoantibody response to tissue-derived native antigens. Joshua R. Ehrlich, Liangdan Tang, Robert J. Caiazzo Jr., Daniel W. Cramer, Shu-Kay Ng, Shu-Wing Ng, and Brian C-S Liu 13. Biorepository Standards and Protocols for Collecting, Processing, and Storing Human Tissues. Dean Troyer