Hedgehog Signaling Protocols

TABLE OF CONTENTSChapter 1. Purifying the Hedgehog protein and its variants by Darren P.Baker, Frederick R. Taylor and R. Blake PepinskyChapter 2. Application of Sonic hedgehog to the developing chick limbby Eva Tiecke and Cheryll TickleChapter 3. Manipulation of Hedgehog signaling in Xenopus by means ofembryo microinjection and application of chemical inhibitors by ThomasHollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas PielerChapter 4. Isolation of rat telencephalic neural explants to assay ShhGABAergic interneuron differentiation-inducing activity by Rina Madyand Jhumku D. KohtzChapter 5. Genetic Analysis of the Vertebrate Hedgehog SignalingPathway Using Muscle Cell Fate Specification in the Zebrafish Embryo bySudipto RoyChapter 6. Efficient Manipulation of Hedgehog/GLI Signaling usingRetroviral Expression Systems by Maria Kasper, Gerhard Regl, ThomasEichberger, Anna-Maria Frischauf and Fritz AbergerChapter 7. Cell Surface Marker and Cell Cycle Analysis, HedgehogSignaling, and Flow Cytometry by Kristina Detmer and Ronald E. GarnerChapter 8. Detecting tagged Hedgehog with Intracellular andExtracellular Immunocytochemistry for functional analysis by AinhoaCallejo, Luis Quijada and Isabel GuerreroChapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupledwith Fluorescent in situ Hybridization of Hedgehog Target Genes byArmel Gallet and Pascal P. ThérondChapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vectorfor temporally and spatially controlled RNAi in Drosophila by EricMarois and Suzanne EatonChapter 11. Germline clone analysis for maternally acting DrosophilaHedgehog components by Erica M. Selva and Beth E. StronachChapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatictissues by Christine M. Bankers and Joan E. HooperChapter 13. GAL4/UAS targeted gene expression for studyingDrosophilaHedgehog signaling by Denise Busson and Anne-Marie PretChapter 14. Biochemical Fractionation of Drosophila Cells by MelanieStegman and David RobbinsChapter 15. Using Immunoprecipitation to Study Protein-proteinInteractions in the Hedgehog Signaling Pathway by Chao Tong and JinJiangChapter 16. Sequence analyses to study the evolutionary history andcis-regulatory elements of Hedgehog genes by Ferenc Müller andAnne-Gaelle Borycki