Patch-Clamp Methods and Protocols

Table of contentsPart 1. Pharmacology1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDAreceptors using whole-cell patch clamp method. Laszlo Fodor and JozsefNagy2. Memantine as an example of a fast, voltage-dependent, open channelN-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and KateGilling3. Methods for evaluation of positive allosteric modulators ofglutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and EricS. Nisenbaum4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia andJia Xu5. Flip-the-Tip: automated patch clamping based on glass electrodes.Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter andAlbrecht Lepple-Wienhues6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes.Christine Leisgen, Mike Kuester and Christoph MethfesselPart 2. Physiology7. Infrared guided laser stimulation as a tool for elucidating thesynaptic site of expression of long-term synaptic plasticity. GerhardRammes, Mathias Eder, Walter Zieglgänsberger and Hans-Ulrich Dodt8. Single-cell RT-PCR, a Technique to Decipher the Electrical,Anatomical and Genetic Determinants of Neuronal Diversity. MariaToledo-Rodriguez and Henry Markram9. Mechanosensitive Ion Channels investigated simultaneously byScanning Probe Microscopy and Patch clamp. Matthias G. Langer10. Synaptic Connectivity in Engineered Neuronal Networks. PeterMolnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J.Hickman11. Modeling of Action Potential Generation in NG108-15 Cells. PeterMolnar and James J. Hickman12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with theSilicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, BrunoAllard and Vincent Jacquemond13. Determination of channel properties at the unitary level in adultmammalian isolated cardiomyocytes. RomainGuinamard14. Electrophysiological Properties of Embryonic Stem Cells DuringDifferentiation into Cardiomyocyte-like Cell Types. Antoni C.G. vanGinneken and Arnoud. C. Fijnvandraat15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. DorvalII, Jonathan Bettencourt, Theoden I. Netoff and John A. White16. Cardiac channelopathies studied with the dynamic action potentialclamp technique. Géza Berecki, Jan G. Zegers, Ronald Wilders and AntoniC.G. van GinnekenPart 3. Biophysics17. Principles of Single-Channel Kinetic Analysis. Feng Qin *Theoretical chapter18. Use of Xenopus oocytes to measure ionic selectivity of pore-formingpeptides and ion channels. Thierry Cens and Pierre Charnet19. Estimation of quantal parameters with multiple-probabilityfluctuation analysis. Chiara Saviane and Robin Angus Silver