Free Radicals and Antioxidant Protocols

ContentsPreface Contributors Part I Reactive Oxygen and Nitrogen Species1. In Vivo Imaging of Free Radicals and OxygenDeepti S. Vikram, Brian K. Rivera, and Periannan Kuppusamy 2 . In Vivo Measurement of Tissue Oxygen using Electron Paramagnetic Resonance Spectroscopy with Oxygen-sensitive Paramagnetic Particle, Lithium Phthalocyanine F. Hyodo, S. Matsumoto, E. Hyodo, A. Matsumoto, K. Matsumoto, and M. C. Krishna3. Measurement of Plasma Nitrite by Chemiluminescence Enika Nagababu and Joseph M Rifkind4. Determination of Glutathione, Mitochondrial Transmembrane Potential, and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen SpeciesK. Sathishkumar, Xueli Gao, Achuthan C. Raghavamenon, Narasimham L. Parinandi, and Rao M. UppuPart II Natural and Synthetic Antioxidants 5. Phenolic Acids and Flavonoids: Ocurrence and Analytical MethodsConstantine D. Stalikas6. Design, Synthesis, and Action of Antiatherogenic AntioxidantsOsamu Cynshi, Kunio Tamura, and Etsuo Niki 7. Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity against Lipid Peroxidation Adriana R. Pohlmann, Scheila Rezende Schaffazick, Tânia B. Creczynski-Pasa, and Sílvia S. Guterres8. Nanoparticle and Iron Chelators as a Potential Novel Alzheimer TherapyGang Liu, Ping Men, George Perry, and Mark A. Smith 9. Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes Eric Simone, Thomas Dziubla , Vladimir Shuvaev, and Vladimir R. Muzykantov10. Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo Enika Nagababu, Joseph M. Rifkind, Sesikeran Boindala, andLakshmaiah NakkaPart III Cellular Oxidative Stress 11. The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or HyperoxiaDouglas R. Spitz and Shannon J. Sullivan 12. A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid SignalingMichelle A. Kline, E. S. O'Connor Butler, Adam Hinzey, Sean Sliman, Clay B. Marsh, Rao M. Uppu, and Narasimham L. Parinandi 13. Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile DysfunctionW. Deng , T.J. Bivalacqua , W. Hellstrom , S. N. Murthy , and Philip J. Kadowitz14. Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic AcidsJames M. May15. Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere ShorteningMuthuswamy Balasubramanyam, Antonysunil Adaikalakoteswari, Zaheer Sameermahmood, and Viswanathan Mohan16. Simultaneous Analysis of Mutiple Redox-Sensitive and Apoptotic Gene Expression in Hypothalamic Neurons Exposed to Cholesterol Ozonation ProductsK.Sathishkumar, Achuthan C. Raghavamenon, Karunakaran Ganeshkumar, Rameshwari Telaprolu and Rao M. Uppu17. Redox Homeostasis and Cellular Stress Response in Aging and NeurodegenerationVittorio Calabrese, Cesare Mancuso, Riccardo Lentile, A.M. Giuffrida Stella, and D. Allan Butterfield.18. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary HypertensionW. Deng, T.J. Bivalacqua, W. Hellstrom, Subramanyam N. Murthy, and Philip J. Kadowitz Part IV DNA Oxidation,