Mouse Cell Culture: Methods and Protocols

1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid BodiesMelany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) CulturesAlastair S. Garfield3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem CellsJanet Smith and Deborah Merrick4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA InterferenceJamie A. Davies5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular DifferentiationPierre-Marie del Moral and David Warburton6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and IntestineJonathan M. Quinlan, Wei-Yuan Yu, and David Tosh7. Isolation and Culture of Embryonic Pancreas and LiverZoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh8. Isolation and Culture of Mouse Satellite CellsAntonio Musarò and Laura Barberi9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte CulturesNigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine KeratinocytesReto Caldelari and Eliane J. Müller11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial CellsMatthew J. Smalley12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent ProbesMagalie A. Ravier and Guy A. Rutter13. Isolation and Culture of Adult Mouse HepatocytesWan-ChunLi, Kate L. Ralphs, and David Tosh14. Isolation and Culture of Mouse Intestinal CellsF. Charles Campbell15. Derivation of Primary Choroid Plexus Epithelial Cells from the MouseTrevelyan R. Menheniott, Marika Charalambous, and Andrew Ward16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent CytochemistryCarla Sciarretta and Liliana Minichiello17. Cell Culture of Primary Cerebellar Granule CellsDana Krämer and Liliana Minichiello18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse BrainHenrik Ahlenius and Zaal Kokaia