The Nucleus: Volume 1: Nuclei and Subnuclear Components

I. THE INTRANUCLEAR ENVIRONMENT 1. The intranuclear environment. Santiago Schnell and Ronald Hancock II. ISOLATION OF NUCLEI 2. Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle. Gavin S. Wilkie and Eric C. Schirmer 3. Isolation of Highly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure. Joseph C. Reese, Hesheng Zhang and Zhengjian Zhang 4. Working with oocyte nuclei: cytological preparations of active chromatin and nuclear bodies from amphibian germinal vesicles. Garry T. Morgan. 5. Preparation of Arabidopsis Nuclei and Nucleoli. Peter McKeown, Alison F. Pendle and Peter J. Shaw 6. High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures from Trypanosomes. Jeffrey A. DeGrasse, Brian T. Chait, Mark C. Field and Michael P. Rout 7. Methods for studying the nuclei and chromosomes of dinoflagellates. Marie-Odile Soyer-Gobillard III. THE NUCLEOLUS 8. Isolation of nucleoli from Ehrlich ascites tumor cells and dynamics of nascent RNA within isolated nucleoli. Marc Thiry and Dominique Ploton 9. Time-lapse microscopy and FRET to analyze the dynamics and interactions of nucleolar proteins in living cells. Emilie Louvet, Marc Tramier, Nicole Angelier and Danièle Hernandez-Verdun 10. Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography. Pavel Tchelidze, Hervé Kaplan, Adrien Beorchia, Marie-Françoise O'Donohue, Hélène Bobichon, Nathalie Lalun, Laurence Wortham and Dominique Ploton IV : INTRANUCLEAR STRUCTURES 11. The perinucleolar compartment (PNC): detection by immunohistochemistry. Alicja Slusarczyk and Sui Huang 12.Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei. Olga V. Zatsepina, Oxana O. Zharskaya and Andrei N. Prusov 13. Isolation of pathology-associated intranuclear inclusions. Christine Iwahashi and Paul J Hagerman 14. The nuclear ubiquitin-proteasome system: visualization of proteasomes, protein aggregates and proteolysis in the cell nucleus. Anna von Mikecz, Min Chen, Thomas Rockel and Andrea Scharf V: INTERPHASE CHROMOSOMES 15. Multicolor 3D-FISH for imaging interphase chromosomes. M. Cremer, F. Grasser, C. Lanctot, S. Müller, M. Neusser, I. Solovei, R. Zinner and T. Cremer 16. Fluorescent transgenes to study interphase chromosomes in living plants. Antonius J.M. Matzke, Bruno Huettel, Johannes van der Winden and Marjori Matzke 17. Analysis of telomeres and telomerase . Jirí Fajkus, Martina Dvorácková and Eva Sýkorová 18. Combined immunofluorescence, RNA FISH, and DNA FISH to study chromatin changes, transcriptional activity, nuclear organization, and X-chromosome inactivation. Julie Chaumeil, Sandrine Augui, Jennifer C. Chow and Edith Heard 19. Analysis of the mobility of DNA double-strand break-containing chromosome domains in living mammalian cells. P.M. Krawczyk, J. Stap, R.A. Hoebe, C.H. van Oven, R. Kanaar, and J.A. Aten