Antibiotic Resistance Protocols: Second Edition

Part I: Epidemiology and Population Genetics1. The RM Test for Determining Methicillin-Resistant Staphylococcus aureus (MRSA) LineagesJodi A. Lindsay and Julia M.-L. Sung2. Unravelling the Ecology of Antibiotic Resistant Bacteria in the NasopharynxBambos M. Charalambous and Ndeky M. OriyoPart II: Genomics and Gene Expression3. Methods to Determine Antibiotic Resistance Gene SilencingVirve I. Enne and Peter M. Bennett4. Studying Gene Induction of Glycopeptide Resistance Using Gene SwappingHee-Jeon Hong5. Real Time PCR Methods to Study Expression of Genes Related to HypermutabilityDenise O'Sullivan6. Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial CompoundsSimon. J. Waddell and Philip D. ButcherPart III: Fitness Mutation and Physiology7. Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase ActivityMirjana Macvanin and Diarmaid Hughes8. Measurements of Heme Levels and Respiration Rate in Salmonella enterica serovar TyphimuriumDiarmaid Hughes and Mirjana Macvanin9. Methods to Determine Fitness in BacteriaCassie F. Pope, Timothy D. McHugh, and Stephen H. Gillespie10. Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosisJoanna Bacon, Kim A. Hatch, and Jon AllnuttPart IV: Detection of Resistance11. Identification of Efflux-Mediated Multi-Drug Resistance in Bacterial Clinical Isolates by Two Simple MethodsMarta Martins, Isabel Couto, Miguel Viveiros, and Leonard Amaral12. Evaluation of Efflux Activity of Bacteria by a Semi-Automated Fluorometric SystemMiguel Viveiros, LilianaRodrigues, Marta Martins, Isabel Couto, Gabriella Spengler, Ana Martins, and Leonard Amaral13. Measuring the Activity of Active Efflux in Gram-Negative BacteriaMark A. Webber and Nick G. Coldham14. Rapid Characterization of b -Lactamases by Multiplex PCRNeil Woodford15. Rapid Methods for Testing Inhibitors of Mycobacterial GrowthDimitrios Evangelopoulos and Sanjib Bhakta16. Use of Biofilm Model Systems to Study Antimicrobial SusceptibilityJonathan Pratten and Derren Ready17. Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®Catherine Arnold