Bioluminescence: Methods and Protocols

1. Luminescent Probes and Visualization of BioluminescenceElisa Michelini, Luca Cevenini, Laura Mezzanotte, and Aldo Roda2. Validation of Bioluminescent Imaging TechniquesJohn Virostko and E. Duco Jansen3. Assessment of Extracellular ATP ConcentrationsLucia Seminario-Vidal, Eduardo R. Lazarowski, and Seiko F. Okada4. High Throughput Quantitative Bioluminescence Imaging for Assessing Tumor BurdenAngelina Contero, Edmond Richer, Ana Gondim, and Ralph P. Mason5. Fluorescence Imaging of Tumors with 'Smart' pH-Activatable Targeted ProbesDaisuke Asanuma, Hisataka Kobayashi, Tetsuo Nagano, and Yasuteru Urano6. Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, B. Christoffer Lagerholm, Alan S. Waggoner, and Marcel P. Bruchez7. Bioluminescent Imaging of Transplanted IsletsXiaojuan Chen and Dixon B. Kaufman8. Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cell Survival, Proliferation, and Fate Kitchener D. Wilson, Mei Huang, and Joseph C. Wu9. Detection of Apoptosis Using Cyclic Luciferase in Living MammalsAkira Kanno, Yoshio Umezawa, and Takeaki Ozawa10. Non-Invasive Bioluminescent Imaging of InfectionsJavier S. Burgos11. Real-Time Bioluminescence Imaging of Viral PathogenesisKathryn E. Luker and Gary D. Luker12. Bioluminescent Monitoring of in vivo Colonization and Clearance Dynamics by Light-Emitting BacteriaSiouxsie Wiles, Brian D. Robertson, Gad Frankel, and Angela Kerton13. Quantitative In Vivo Imaging of Non-Viral Mediated Gene Expressionand RNAi Mediated KnockdownGarrett R. Rettig and Kevin G. Rice14. Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy TransferKevin D.G. Pfleger15. Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene AssayAkira Kanno, Takeaki Ozawa, and Yoshio Umezawa16. Bioluminescence Analysis of Smad-Dependent TGF-b Signaling in Live MiceJian Luo and Tony Wyss-Coray17. Bioluminescence Imaging of Calcium Oscillations Inside Intracellular OrganellesCarlos Villalobos, María Teresa Alonso, and Javier García-Sancho18. Novel Tools for Use in Bioluminescence Resonance Energy Transfer (BRET) AssaysMélanie Robitaille, Isabelle Héroux, Alessandra Baragli, and Terence E. Hébert19. PIN-G Reporter for Imaging and Defining Trafficking Signals in Membrane ProteinsLynn Mckeown, Vicky C. Jones, and Owen T. Jones20. Imaging ß-Galactosidase Activity in vivo Using Sequential Reporter-Enzyme LuminescenceGeorges von Degenfeld, Tom S. Wehrman, and Helen M. Blau