Clostridium difficile: Methods and Protocols

Part I: Introduction to C. difficile and the Disease It Causes1. Clostridium difficile: No Longer an Enigmatic Pathogen?Adam P. Roberts and Peter Mullany2. Clostridium difficile and the Disease It CausesTorbjörn NorénPart II: Isolation and Culture Techniques3. Clostridium difficile Isolation and Culture TechniquesMike WrenPart III: Methods for Typing and Epidemiological Studies4. Molecular Typing Methods for C. difficile: Pulsed-Field Gel Electrophoresis and PCR RibotypingSandra Janezic and Maja Rupnik5. Clostridium difficile ToxinotypingMaja Rupnik6. Multilocus Sequence Typing for Clostridium difficileLudovic Lemée and Jean-Louis PonsPart IV: Biochemistry of the Organism7. Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin GenesAna Antunes and Bruno Dupuy8. Dissecting the Cell SurfaceRobert Fagan and Neil Fairweather9. Human Intestinal Epithelial Response(s) to Clostridium difficileNazila V. Jafari, Elaine Allan, and Mona Bajaj-ElliottPart V: Genomics10. Comparative Genome Analysis of Clostridium difficile Using DNA MicroarraysRichard Stabler, Lisa Dawson, and Brendan WrenPart VI: Development of Systems for Genetic Analysis of the Organism11. ClosTron-Targeted MutagenesisJohn T. Heap, Stephen T. Cartman, Sarah A. Kuehne, Clare Cooksley, and Nigel P. Minton12. Methods for Gene Cloning and Targeted MutagenesisGlen P. Carter, Dena Lyras, Rachael Poon, Pauline M. Howarth, and Julian I. Rood13. Transposon Mutagenesis in C. difficileHaitham A. Hussain, Adam P. Roberts, Rachael Whalan, and Peter MullanyPart VII: Animal Models ofDisease14. Refinement of the Hamster Model of Clostridium difficile DiseaseGillian Douce and David Gouding15. Methods for Working with the Mouse ModelAnne Collignon