Mitosis: Methods and Protocols

1. Knock-In and Knock-Out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic PathwaysAlexis R. Barr, Deborah Zyss, and Fanni Gergely2. Functional Dissection of Mitotic Regulators through Gene Targeting in Human Somatic CellsEli Berdougo, Marie-Emilie Terret, and Prasad V. Jallepalli3. RNAi in Drosophila S2 Cells As A Tool For Studying Cell Cycle ProgressionMónica Bettencourt-Dias and Gohta Goshima4. Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding YeastMarco Geymonat, Adonis Spanos, and Steven Sedgwick5. Hydrodynamic Analysis of Human Kinetochore Complexes during MitosisSarah E. McClelland and Andrew D. McAinsh6. Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured CellsPier Paolo D'Avino, Vincent Archambault, Marcin R. Przewloka, Wei Zhang, Ernest D. Laue, and David M. Glover7. Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell LinesMichael H. A. Schmitz and Daniel W. Gerlich8. Electron Tomography of Microtubule End-Morphologies in C. elegans EmbryosEileen O'Toole and Thomas Müller-Reichert9. Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila CellsAntónio J. Pereira, Irina Matos, Mariana Lince-Faria, and Helder Maiato10. Fluorescence Imaging of the Centrosome Cycle in Mammalian CellsSuzanna L. Prosser and Andrew M. Fry11. Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin under the Native PromoterMasamitsu Sato, Mika Toya, and Takashi Toda12. Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome CongressionChristiane Klebig, Alberto Toso,Satyarebala Borusu, and Patrick Meraldi13. Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg ExtractsMichael J. Emanuele and P. Todd Stukenberg14. Live Cell Imaging of Kinetochore Capture by Microtubules in Budding YeastKozo Tanaka and Tomoyuki U. Tanaka15. The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and RecoveryJosefin Fernius and Kevin G. Hardwick16. Measuring Proteolysis in MitosisCatherine Lindon and Barbara Di Fiore17. An In Vitro Assay for Cdc20-dependent Mitotic Anaphase-Promoting Complex Activity from Budding YeastScott C. Schuyler and Andrew W. Murray18. In vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg ExtractsHiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, and Yuu Kimata19. Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and DegradationAdam Williamson, Lingyan Jin, and Michael Rape20. Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive ('Pseudosubstrate') InhibitorsMatthew K. Summers and Peter K. Jackson