Heterologous Expression of Membrane Proteins: Methods and Protocols

Heterologous Expression of Membrane Proteins: Methods and ProtocolsIsabelle Mus-Veteau, Editor Table of ContentsPrefaceContributors1. Heterologous expression of membrane proteins for structural analysis Isabelle Mus-Veteau2. Production of membrane proteins in E. coli and L. lactisBert Poolman and Eric R. Geertsma3. Mammalian membrane receptors expression as inclusion bodies in E. coliBernard Mouillac and Jean-Louis Banères 4. Expression of membrane proteins at the E. coli membrane for structural studiesManuela Zoonens and Bruno Miroux5. Membrane protein expression in Lactococcus lactisAnnie Frelet-Barrand, Sylvain Boutigny, Edmund R. S. Kunji and Norbert Rolland6. Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiaeOlivier Joubert, Rony Nehme, Michel Bidet and Isabelle Mus-Veteau7. Mammalian membrane proteins expression in baculovirus infected insect cellsCéline Trometer and Pierre Falson8. Expression of membrane proteins in Drosophila melanogaster S2 cells: production and analysis of a EGFP-fused G Protein-Coupled Receptor as a modelKarl Brillet, Carlos A. Pereira, and Renaud Wagner9. Membrane protein expression in the eyes of transgenic flies.Valérie Panneels and Irmgard Sinning10. Expression of Mammalian Membrane Proteins in Mammalian Cells using Semliki Forest Virus VectorsKenneth Lundstrom11. Membrane Protein Expression in Cell-Free SystemsBirgit Schneider, Friederike Junge, Vladimir A. Shirokov, Florian Durst, Daniel Schwarz, Volker Dötsch, Frank Bernhard12. Practical considerations of membrane proteininstability for purification and crystallisation Christopher G. Tate13. Membrane protein solubilizationKatia Duquesne and James N. Sturgis 14. Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitroCécile Breyton, Bernard Pucci and Jean-Luc Popot15. Heterologous expression and affinity purification of eukaryotic membrane proteins in view of functional and structural studies: the example of the sarcoplasmic reticulum Ca2+-ATPaseDelphine Cardi, Cédric Montigny, Bertrand Arnou, Marie Jidenko, Estelle Marchal, Marc le Maire and Christine Jaxel