Peptidomics: Methods and Protocols

Part I: Introduction1. Peptidomics: Divide et ImperaMikhail SolovievPart II: From Bacteria to Men2. Performing Comparative Peptidomics Analyses of Salmonella from Different Growth ConditionsJoshua N. Adkins, Heather Mottaz, Thomas O. Metz, Charles Ansong, Nathan P. Manes, Richard D. Smith, and Fred Heffron3. Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegansSteven J. Husson, Elke Clynen, Kurt Boonen, Tom Janssen, Marleen Lindemans, Geert Baggerman, and Liliane Schoofs4. Mass Spectrometric Analysis of Molluscan NeuropeptidesKa Wan Li and August B. Smit5. Monitoring Neuropeptides in vivo via Microdialysis and Mass SpectrometryHeidi L. Behrens and Lingjun Li6. Protocols for Peptidomic Analysis of Spider Venoms Liang Songping7. Purification and Characterization of Biologically Active Peptides from Spider VenomsAlexander A. Vassilevski, Sergey A. Kozlov, Tsezi A. Egorov, and Eugene V. Grishin8. MALDI-TOF Mass Spectrometry Approaches to the Characterization of Insect NeuropeptidesRobert J. Weaver and Neil Audsley9. Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of DrosophilaChristian Wegener, Susanne Neupert, and Reinhard Predel10. Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass SpectrometryJoachim Schachtner, Christian Wegener, Susanne Neupert, and Reinhard Predel11. Peptidomic Analysis of Single Identified NeuronsSusanne Neupert and Reinhard Predel12. Identification and Analysis of Bioactive Peptides in Amphibian Skin SecretionsJ. Michael Conlon and Jérôme Leprince13. An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial PeptidecDNAsShawichi Iwamuro and Tetsuya Kobayashi14. Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial PeptidesRen Lai15. Identification and Relative Quantification of Neuropeptides from the Endocrine TissuesKurt Boonen, Steven J. Husson, Bart Landuyt, Geert Baggerman, Eisuke Hayakawa, Walter H.M.L. Luyten, and Liliane Schoofs16. Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography PrefractionationLianghai Hu, Mingliang Ye, and Hanfa Zou17. Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mass Spectrometric AnalysisRobert M. Sturm, James A. Dowell, and Lingjun Li18. Quantitative Neuroproteomics of the SynapseDinah Lee Ramos-Ortolaza, Ittai Bushlin, Noura Abul-Husn, Suresh P. Annagudi, Jonathan Sweedler, and Lakshmi A. Devi19. Peptidomics Analysis of Lymphoblastoid Cell LinesAnne Fogli and Philippe Bulet20. Peptidomics: Identification of Pathogenic and Marker PeptidesYang Xiang, Manae S. Kurokawa, Mie Kanke, Yukiko Takakuwa, and Tomohiro KatoPart III: Tools and Approaches21. Peptidomic Approaches to the Identification and Characterization of Functional Peptides in HydraToshio Takahashi and Toshitaka Fujisawa22. Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and their PrecursorsNigel M. Page and Nicola J. Weston-Bell23. Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and MicroarraysFan Zhang, Anna Dulneva, Julian Bailes, and Mikhail Soloviev24. In situ Biosynthesis of Peptide ArraysMingyue He and Oda Stoevesandt25. Bioinformatic Approaches to the Identification of Novel Neuropeptide PrecursorsElke Clynen, Feng