Plant Stress Tolerance: Methods and Protocols

Part I: Reviews and Overview Chapters1. Dehydration Tolerance in PlantsMelvin J. Oliver, John C. Cushman, and Karen L. Koster2. Approaches to Identifying Genes for Salinity Tolerance, and the Importance of Time ScaleRana Munns3. Gene Regulation During Cold Stress Acclimation in PlantsViswanathan Chinnusamy, Jian-Kang Zhu, and Ramanjulu Sunkar4. Redox-Dependent Regulation, Redox Control, and Oxidative Damage in Plant Cells Subjected to Abiotic StressKarl-Josef Dietz5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic StressNese Sreenivasulu, Ramanjulu Sunkar, Ulrich Wobus, and Marc Strickert6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in PlantsAnanda Mustafiz, Khirod K. Sahoo, Sneh L. Singla-Pareek, and Sudhir K. SoporyPart II: Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs7. Genetic Screens to Identify Plant Stress GenesCsaba Papdi, Jeffrey Leung, Mary Prathiba Joseph, Imma Perez Salamó, and László Szabados8. Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive GenesAkihiro Matsui, Junko Ishida, Taeko Morosawa, Masanori Okamoto, Jong-Myong Kim, Yukio Kurihara, Makiko Kawashima, Maho Tanaka, Taiko Kim To, Kentaro Nakaminami, Eli Kaminuma, Takaho A. Endo, Yoshiki Mochizuki, Shuji Kawaguchi, Norio Kobayashi, Kazuo Shinozaki, Tetsuro Toyoda, and Motoaki Seki9. Identification of Stress Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an ExampleLila Peal, Michael Puckette, and Ramamurthy Mahalingam10. Identification of DNA-Binding Proteins and Protein-Protein-Interactions by Yeast One Hybrid and Yeast Two HybridScreeningPeter Klein and Karl-Josef Dietz11. Functional Characterization of Water Deficit Stress-Induced Genes Using RNAiMuthappa Senthil-Kumar, Makarla Udayakumar, and Kirankumar S. Mysore12. Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated ProteinsJenny Renaut13. Thiol-Disulfide Redox Proteomics in Plant ResearchMeenakumari Muthuramalingam, Karl-Josef Dietz, and Elke Ströher14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from PlantsJose Luis Reyes, Catalina Arenas-Huertero, and Ramanjulu Sunkar15. An Array Platform for Identification of Stress-Responsive MicroRNAs in PlantsXiaoyun Jia, Venugopal Mendu, and Guiliang TangPart III: Antioxidant Enzymes and Metabolites16. Spectrophotometric Assays for Antioxidant Enzymes in PlantsSathya Elavarthi and Bjorn Martin17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde DehydrogenasesHans-Hubert Kirch and Horst Röhrig18. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in PlantsNiranjani JambunathanPart IV: Osmotic Adjustment and Ion Measurements19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic StudiesPaul E. Verslues20. Methods for Determination of Proline in PlantsEdit Ábrahám, Cecile Hourton-Cabassa, László Erdei, and László Szabados21. A New Method for Accurately Measuring Delta1-Pyrroline-5-Carboxylate Synthetase ActivityElodie Parre, Jacques de Virville, Françoise Cochet, Anne-Sophie Leprince, Luc Richard, Delphine Lefebvre-De Vos, Mohamed Ali Ghars, Marianne