Transcription Factors: Methods and Protocols

Table of ContentsPrefaceContributorsPart I. Transcription Factor Trafficking and Post-Translational Modifications: Overviews of Mechanisms1. A review of post-translational modifications and sub-cellular localization of Etstranscription factors: possible connection with cancer and involvement in the hypoxic reponseCeline Charlot, Helene Dubois-Pot, Tsvetan Serchov, Yves TouretteAnd Bohdan Wasylyk2. Regulation of transcription factor function by targeted protein degradation: an overview focusing on p53, c-Myc and c-junJukka Westermarck3. Review of molecular mechanisms involved in the activation of the Nrf2-AREsignaling pathway by chemopreventive agentsAldo Giudice, Claudio Arra and Maria C. Turco Part II. Cytoplasmic-nuclear trafficking of transcription factors4. Subnuclear localization and intranuclear trafficking of transcription factorsSayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, RachitBakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein5. Analysis of ligand dependent nuclear accumulation of Smads in TGF-b signalingDouglas A. Chapnick and Xuedong Liu6. Raf/MEK/MAPK signaling stimulates the nuclear translocation and trans-activating activity of FOXM1Richard Y.M. Ma, Tommy H.K. Tong, Wai Ying Leung and Kwok-Ming Yao7. Coupling of dephosphorylation and nuclear export of Smads in TGF-b signalingFangyan Dai, Xueyan Duan, Yao-Yun Liang, Xia Lin and Xin-Hua Feng 8. Assessing sequence-specific DNA binding and transcriptional activity of STAT1 transcription factorThomas Meyer and Uwe Vinkemeier9. Analysis of nuclear export using photoactivatable GFP fusion proteins andinterspecies heterokaryonsKerry-Ann Nakrieko, Iordanka A. Ivanova and Lina Dagnino10. Determination of nuclear localization signal sequences for Kruppel-like factor 8Tina S. Mehta, Farah Monzur and Jihe Zhao11. Methods to measure nuclear export of beta-catenin using fixed and live cell assaysManisha Sharma and Beric R. Henderson12. Imaging of transcription factor trafficking in living cells: lessons fromcorticosteroid receptor dynamicsMayumi Nishi Part III. Post-Translational Modifications and Impact on Function13. Hypoxia-inducible factors: posttranslational crosstalk of signalling pathwaysElitsa Y. Dimova and Thomas Kiezmann14. The basic helix-loop-helix leucine-zipper gene Mitf: analysis of alternativepromoter choice and splicingKapil Bharti, Julien Debbache, Xin Wang and Heinz Arnheiter15. Phosphorylation control of nuclear receptorsSebastien Lalevee, Christine Ferry and Cecile Rochette-Egly16. Regulation of Krüpple-like factor 5 by targeted protein degradationCeshi Chen.17. Post-translational control of ETS transcription factors: detection of modifiedfactors at target gene promotersLi Li, Janice Saxton and Pete E. Shaw18. Integration of protein kinases into transcription complexes: identifyingcomponents of immobilized in vitro pre-initiation complexes Hong-Mei Zhang, Stephanie Vouier, Glenn Hodgson and Peter E, Shaw19. Posttranslational modification of p53 by ubiquitinChunhong Yan20. Phosphorylation-dependent