Photosynthesis Research Protocols

1. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher PlantsYasusi Yamamoto, Jing Leng, and Jian-Ren Shen2. Isolation of Photosystem I Submembrane FractionsJohanne Harnois, Najoua Msilini, and Robert Carpentier3. Isolation of Photosystem II Reaction Center Complexes from PlantsMichael Seibert and Rafael Picorel4. Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803Dmitrii V. Vavilin5. Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic CyanobacteriaJian-Ren Shen, Keisuke Kawakami, and Hiroyuki Koike6. Isolation of Cytochrome b6f Complex from Grana and Stroma Membranes from Spinach ChloroplastsElzbieta Romanowska7. Purification and Crystallization of the Cytochrome b6f ComplexDanas Baniulis, Huamin Zhang, Taisaya Zakharova, S. Saif Hasan, and William A. Cramer8. Purification of Plastocyanin and Cytochrome c6 from Plants, Green Algae, and CyanobacteriaJosé A. Navarro, Manuel Hervás, and Miguel A. De la Rosa9. Isolation and Identification of Chloroplast LipidsNorihiro Sato and Mikio Tsuzuki10. Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from PlantsRafael Picorel, Miguel Alfonso, and Michael Seibert11. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b ComplexWolfgang Rühle and Harald Paulsen12. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies with Light-Inducible Structural TransitionsIlian Simidjiev, Zsuzsanna Várkonyi, Petar H. Lambrev, and Gyözö Garab13. Protein Targeting across and into Chloroplast MembranesSteven M. Theg and Shari M. Lo14. Proteomic Analysis of Thylakoid MembranesVenkateswarlu Yadavalli, Sreedhar Nellaepalli, and Rajagopal Subramanyam15. Thylakoid Phosphoproteins: Identification of Phosphorylation SitesAnne Rokka, Eva-Mari Aro, and Alexander V. Vener16. Direct Detection of Free Radicals and Reactive Oxygen Species in ThylakoidsÉva Hideg, Tamás Kálai, and Kálmán Hideg17. Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher PlantsNobuyoshi Nijo, Björn Lundin, Miho Yoshioka, Noriko Morita, and Yasusi Yamamoto18. Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure-Function CharacterizationDavid Joly, Sridharan Govindachary, and Mário Fragata19. Physical and Chemical Immobilization Methods of Photosynthetic MaterialsLise Barthelmebs, Robert Carpentier, and Régis Rouillon20. Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thalianaVerónica Albrecht, Gonzalo M. Estavillo, Abby J. Cuttriss, and Barry J. Pogson21. Expression of Genes in Cyanobacteria: Adaptation of Endogenous Plasmids as Platforms for High-Level Gene Expression in Synechococcus sp. PCC 7002Yu Xu, Richard M. Alvey, Patrick O. Byrne, Joel E. Graham, Gaozhong Shen, and Donald A. Bryant22. Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803Julian J. Eaton-Rye23. A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtiiVellupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber24. Rapid Isolation of Intact Chloroplasts from Spinach LeavesDavid Joly and Robert Carpentier25. Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 GrassesElzbieta Romanowska and Eugeniusz Parys26. Isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from LeavesA. Elizabete Carmo-Silva, Csengele Barta, and Michael E. Salvucci27. Quantifying the Amount and Activity of Rubisco in LeavesDavid S. Kubien, Christopher M. Brown, and Heather J. Kane28. Purification of Rubisco Activase from Leaves or after Expression in Escherichia coliCsengele Barta, A. Elizabete Carmo-Silva, and Michael E. Salvucci29. Rubisco Activase Activity AssaysCsengele Barta, A. Elizabete Carmo-Silva, and Michael E. Salvucci30. Quantification of Rubisco Activase Content in Leaf ExtractsWataru Yamori and Susanne von Caemmerer